Počet záznamů: 1
Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
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SYSNO ASEP 0563714 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis Tvůrce(i) Iegorova, Viktoriia (BTO-N)
Naraine, Ravindra (BTO-N)
Pšenička, M. (CZ)
Zelazowska, M. (PL)
Šindelka, Radek (BTO-N) RIDCelkový počet autorů 5 Číslo článku 982732 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 10, SEP 20 2022 (2022)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova oogenesis ; RNA localization ; Xenopus laevis ; Acipenser ruthenus ; TOMO-Seq Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA19-11313S GA ČR - Grantová agentura ČR Výzkumná infrastruktura CENAKVA - 90099 - Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000875433000001 EID SCOPUS 85139202925 DOI 10.3389/fcell.2022.982732 Anotace The oocyte is a unique cell, from which develops a complex organism comprising of germ layers, tissues and organs. In some vertebrate species it is known that the asymmetrical localization of biomolecules within the oocyte is what drives the spatial differentiation of the daughter cells required for embryogenesis. This asymmetry is first established to produce an animal-vegetal (A-V) axis which reflects the future specification of the ectoderm, mesoderm, and endoderm layers. Several pathways for localization of vegetal maternal transcripts have already been described using a few animal models. However, there is limited information about transcripts that are localized to the animal pole, even though there is accumulating evidence indicating its active establishment. Here, we performed comparative TOMO-Seq analysis on two holoblastic cleavage models: Xenopus laevis and Acipenser ruthenus oocytes during oogenesis. We found that there were many transcripts that have a temporal preference for the establishment of localization. In both models, we observed vegetal transcript gradients that were established during either the early or late oogenesis stages and transcripts that started their localization during the early stages but became more pronounced during the later stages. We found that some animal gradients were already established during the early stages, however the majority were formed during the later stages of oogenesis. Some of these temporally localized transcripts were conserved between the models, while others were species specific. Additionally, temporal de novo transcription and also degradation of transcripts within the oocyte were observed, pointing to an active remodeling of the maternal RNA pool. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2023 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2022.982732/full
Počet záznamů: 1