Počet záznamů: 1
The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms Including Interference with Auxin Homeostasis
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SYSNO ASEP 0561528 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms Including Interference with Auxin Homeostasis Tvůrce(i) Nakabayashi, K. (GB)
Walker, M. (US)
Irwin, D. (DE)
Cohn, J. (US)
Guida-English, S. M. (US)
Garcia, L. (AR)
Pavlović, Iva (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Tarkowská, Danuše (UEB-Q) RID, ORCID
Strnad, Miroslav (UEB-Q) RID, ORCID
Pérez, M. (ES)
Seville, A. (GB)
Stock, D. (GB)
Leubner-Metzger, Gerhard (UEB-Q) ORCIDCelkový počet autorů 14 Číslo článku 4618 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 9 (2022)Poč.str. 35 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova allelochemical and allelopathy ; aquaporin-mediated water transport ; ATP-binding cassette (ABC) transporter ; auxin transport and homeostasis ; cis-(+)-12-ox-ophytodienoic acid (OPDA) reductase ; gibberellin metabolism ; phytotoxin detoxification programme ; PIN auxin efflux carrier ; seed germination ; WRKY transcription factors Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000800719300001 EID SCOPUS 85128598104 DOI 10.3390/ijms23094618 Anotace Molecular responses of plants to natural phytotoxins comprise more general and com-pound-specific mechanisms. How phytotoxic chalcones and other flavonoids inhibit seedling growth was widely studied, but how they interfere with seed germination is largely unknown. The dihydrochalcone and putative allelochemical myrigalone A (MyA) inhibits seed germination and seedling growth. Transcriptome (RNAseq) and hormone analyses of Lepidium sativum seed responses to MyA were compared to other bioactive and inactive compounds. MyA treatment of im-bibed seeds triggered the phased induction of a detoxification programme, altered gibberellin, cis- (+)-12-oxophytodienoic acid and jasmonate metabolism, and affected the expression of hormone transporter genes. The MyA-mediated inhibition involved interference with the antioxidant system, oxidative signalling, aquaporins and water uptake, but not uncoupling of oxidative phosphoryla-tion or p-hydroxyphenylpyruvate dioxygenase expression/activity. MyA specifically affected the expression of auxin-related signalling genes, and various transporter genes, including for auxin transport (PIN7, ABCG37, ABCG4, WAT1). Responses to auxin-specific inhibitors further supported the conclusion that MyA interferes with auxin homeostasis during seed germination. Comparative analysis of MyA and other phytotoxins revealed differences in the specific regulatory mechanisms and auxin transporter genes targeted to interfere with auxin homestasis. We conclude that MyA exerts its phytotoxic activity by multiple auxin-dependent and independent molecular mechanisms. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa https://doi.org/10.3390/ijms23094618
Počet záznamů: 1