Počet záznamů: 1
Genomic analysis of Acinetobacter pittii CEP14 reveals its extensive biodegradation capabilities, including cometabolic degradation of cis-1,2-dichloroethene
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SYSNO ASEP 0559438 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genomic analysis of Acinetobacter pittii CEP14 reveals its extensive biodegradation capabilities, including cometabolic degradation of cis-1,2-dichloroethene Tvůrce(i) Desmarais, M. (CZ)
Fraraccio, S. (CZ)
Dolinová, I. (CZ)
Rídl, Jakub (UMG-J) ORCID
Strnad, Hynek (UMG-J) RID
Kubátová, H. (CZ)
Ševců, A. (CZ)
Šuman, J. (CZ)
Strejček, M. (CZ)
Uhlík, O. (CZ)Celkový počet autorů 10 Zdroj.dok. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. - : Springer - ISSN 0003-6072
Roč. 115, č. 8 (2022), s. 1041-1057Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Acinetobacter pittii ; Chlorinated ethenes (CEs) ; cis-1 ; 2-dichloroethene (cDCE) biodegradation ; Cometabolism ; Whole-genome shotgun sequencing ; Oxygenase ; Phenol Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Microbiology CEP LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UMG-J - RVO:68378050 UT WOS 000810824300001 DOI 10.1007/s10482-022-01752-6 Anotace Halogenated organic compounds are naturally occurring in subsurface environments, however, accumulation of the degradative intermediate cis-1,2-dichloroethene (cDCE) at soil and groundwater sites contaminated with xenobiotic chlorinated ethenes is a global environmental and public health issue. Identifying microorganisms capable of cDCE degradation in these environments is of interest because of their potential application to bioremediation techniques. In this study, we sequenced, assembled, and analyzed the complete genome of Acinetobacter pittii CEP14, a strain isolated from chloroethene-contaminated groundwater, that has demonstrated the ability for aerobic cometabolic degradation of cDCE in the presence of n-hexane, phenol, and toluene. The A. pittii CEP14 genome consists of a 3.93 Mbp-long chromosome (GenBank accession no. CP084921) with a GC content of 38.9% and three plasmids (GenBank accession no. CP084922, CP084923, and CP084924). Gene function was assigned to 83.4% of the 3,930 coding DNA sequences. Functional annotation of the genome revealed that the CEP14 strain possessed all genetic elements to mediate the degradation of a range of aliphatic and aromatic compounds, including n-hexane and phenol. In addition, it harbors gene clusters involved in cytosol detoxification and oxidative stress resistance, which could play a role in the mitigation of toxic chemical intermediates that can arise during the degradation of cDCE. Gene clusters for heavy metal and antibiotic resistance were also identified in the genome of CEP14. These results suggest that CEP14 may be a versatile degrader of xenobiotic compounds and well-adapted to polluted environments, where a combination of heavy metal and organic compound pollution is often found. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://link.springer.com/article/10.1007/s10482-022-01752-6
Počet záznamů: 1