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Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications
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SYSNO ASEP 0559406 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications Tvůrce(i) Vacková, Irena (FGU-C) ORCID, RID
Vavřínová, E. (CZ)
Musílková, Jana (FGU-C) RID, ORCID
Havlas, V. (CZ)
Petrenko, Yuriy (FGU-C) ORCID, RID, SAIČíslo článku 2553 Zdroj.dok. Polymers. - : MDPI
Roč. 14, č. 13 (2022)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova multipotent mesenchymal stromal cells ; Wharton's jelly ; Hyalofast (R) ; hypothermic storage ; paracrine activity Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP NV19-06-00355 GA MZd - Ministerstvo zdravotnictví LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000824092200001 EID SCOPUS 85132991122 DOI 10.3390/polym14132553 Anotace The regulatory requirements in cell processing, in the choice of a biomaterial scaffold and in quality control analysis, have to be followed in the clinical application of tissue-engineered grafts. Confirmation of sterility during quality control studies requires prolonged storage of the cell-based construct. After storage, preservation of the functional properties of the cells is an important prerequisite if the cells are to be used for cell-based tissue therapies. The study presented here shows the generation of 3D constructs based on Wharton's jelly multipotent mesenchymal stromal cells (WJ-MSCs) and the clinically-acceptable HyaloFast (R) scaffold, and the effect of two- and six-day hypothermic storage of 3D cell-based constructs on the functional properties of populated cells. To study the viability, growth, gene expression, and paracrine secretion of WJ-MSCs within the scaffolds before and after storage, xeno-free culture conditions, metabolic, qPCR, and multiplex assays were applied. The WJ-MSCs adhered and proliferated within the 3D HyaloFast (R). Our results show different viability of the cells after the 3D constructs have been stored under mild (25 degrees C) or strong (4 degrees C) hypothermia. At 4 degrees C, the significant decrease of metabolic activity of WJ-MSCs was detected after 2 days of storage, with almost complete cell loss after 6 days. In mild hypothermia (25 degrees C) the decrease in metabolic activity was less remarkable, confirming the suitability of these conditions for cell preservation in 3D environment. The significant changes were detected in gene expression and in the paracrine secretion profile after 2 and 6 days of storage at 25 degrees C. The results presented in this study are important for the rapid transfer of tissue engineering approaches into clinical applications. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2073-4360/14/13/2553
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