Počet záznamů: 1
Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model
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SYSNO ASEP 0556529 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model Tvůrce(i) Chlupáč, Jaroslav (FGU-C) ORCID, RID
Matějka, R. (CZ)
Koňařík, M. (CZ)
Novotný, R. (CZ)
Šimůnková, Z. (CZ)
Mrázová, I. (CZ)
Fabian, O. (CZ)
Zapletal, M. (CZ)
Pulda, Z. (CZ)
Lipenský, J. F. (CZ)
Štěpanovská, J. (CZ)
Hanzálek, K. (CZ)
Brož, Antonín (FGU-C) RID, ORCID, SAI
Novák, T. (CZ)
Lodererová, A. (CZ)
Voska, L. (CZ)
Adla, T. (CZ)
Froněk, J. (CZ)
Rozkot, M. (CZ)
Forostyak, S. (CZ)
Kneppo, P. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Pirk, J. (CZ)Celkový počet autorů 23 Číslo článku 3310 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 6 (2022)Poč.str. 29 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova blood vessel prosthesis ; decellularization ; cell seeding ; tissue engineering ; allograft ; heterograft ; pericardium ; adipose tissue-derived stromal cells ; Wharton's jelly mesenchymal stem cells Obor OECD Cardiac and Cardiovascular systems CEP NV18-02-00422 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000775384600001 EID SCOPUS 85126683388 DOI 10.3390/ijms23063310 Anotace Background: Cardiovascular surgery is confronted by a lack of suitable materials for patch repair. Acellular animal tissues serve as an abundant source of promising biomaterials. The aim of our study was to explore the bio-integration of decellularized or recellularized pericardial matrices in vivo. Methods: Porcine (allograft) and ovine (heterograft, xenograft) pericardia were decellularized using 1% sodium dodecyl sulfate ((1) Allo-decel and (2) Xeno-decel). We used two cell types for pressure-stimulated recellularization in a bioreactor: autologous adipose tissue-derived stromal cells (ASCs) isolated from subcutaneous fat of pigs ((3) Allo-ASC and (4) Xeno-ASC) and allogeneic Wharton's jelly mesenchymal stem cells (WJCs) ((5) Allo-WJC and (6) Xeno-WJC). These six experimental patches were implanted in porcine carotid arteries for one month. For comparison, we also implanted six types of control patches, namely, arterial or venous autografts, expanded polytetrafluoroethylene (ePTFE Propaten(R) Gore(R)), polyethylene terephthalate (PET Vascutek(R)), chemically stabilized bovine pericardium (XenoSure(R)), and detoxified porcine pericardium (BioIntegral(R) NoReact(R)). The grafts were evaluated through the use of flowmetry, angiography, and histological examination. Results: All grafts were well-integrated and patent with no signs of thrombosis, stenosis, or aneurysm. A histological analysis revealed that the arterial autograft resembled a native artery. All other control and experimental patches developed neo-adventitial inflammation (NAI) and neo-intimal hyperplasia (NIH), and the endothelial lining was present. NAI and NIH were most prominent on XenoSure(R) and Xeno-decel and least prominent on NoReact(R). In xenografts, the degree of NIH developed in the following order: Xeno-decel > Xeno-ASC > Xeno-WJC. NAI and patch resorption increased in Allo-ASC and Xeno-ASC and decreased in Allo-WJC and Xeno-WJC. Conclusions: In our setting, pre-implant seeding with ASC or WJC had a modest impact on vascular patch remodeling. However, ASC increased the neo-adventitial inflammatory reaction and patch resorption, suggesting accelerated remodeling. WJC mitigated this response, as well as neo-intimal hyperplasia on xenografts, suggesting immunomodulatory properties. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1422-0067/23/6/3310
Počet záznamů: 1