Počet záznamů: 1  

Impact of Maturation on Myocardial Response to Ischemia and the Effectiveness of Remote Preconditioning in Male Rats

  1. 1.
    SYSNO ASEP0548713
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevImpact of Maturation on Myocardial Response to Ischemia and the Effectiveness of Remote Preconditioning in Male Rats
    Tvůrce(i) Kindernay, L. (SK)
    Farkašová, V. (SK)
    Neckář, Jan (FGU-C) RID, ORCID
    Hrdlička, Jaroslav (FGU-C) ORCID, RID
    Ytrehus, K. (NO)
    Ravingerová, T. (SK)
    Číslo článku11009
    Zdroj.dok.International Journal of Molecular Sciences. - : MDPI
    Roč. 22, č. 20 (2021)
    Poč.str.18 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaischemia/reperfusion injury ; remote ischemic preconditioning ; maturation ; protective cell signaling
    Vědní obor RIVED - Fyziologie
    Obor OECDPhysiology (including cytology)
    CEPNU21J-02-00039 GA MZd - Ministerstvo zdravotnictví
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000715599900001
    EID SCOPUS85116769863
    DOI10.3390/ijms222011009
    AnotaceAging attenuates cardiac tolerance to ischemia/reperfusion (I/R) associated with defects in protective cell signaling, however, the onset of this phenotype has not been completely investigated. This study aimed to compare changes in response to I/R and the effects of remote ischemic preconditioning (RIPC) in the hearts of younger adult (3 months) and mature adult (6 months) male Wistar rats, with changes in selected proteins of protective signaling. Langendorff-perfused hearts were exposed to 30 min I/120 min R without or with prior three cycles of RIPC (pressure cuff inflation/deflation on the hind limb). Infarct size (IS), incidence of ventricular arrhythmias and recovery of contractile function (LVDP) served as the end points. In both age groups, left ventricular tissue samples were collected prior to ischemia (baseline) and after I/R, in non-RIPC controls and in RIPC groups to detect selected pro-survival proteins (Western blot). Maturation did not affect post-ischemic recovery of heart function (Left Ventricular Developed Pressure, LVDP), however, it increased IS and arrhythmogenesis accompanied by decreased levels and activity of several pro-survival proteins and by higher levels of pro-apoptotic proteins in the hearts of elder animals. RIPC reduced the occurrence of reperfusion-induced ventricular arrhythmias, IS and contractile dysfunction in younger animals, and this was preserved in the mature adults. RIPC did not increase phosphorylated protein kinase B (p-Akt)/total Akt ratio, endothelial nitric oxide synthase (eNOS) and protein kinase C epsilon (PKC epsilon) prior to ischemia but only after I/R, while phosphorylated glycogen synthase kinase-3 beta (GSK3 beta) was increased (inactivated) before and after ischemia in both age groups coupled with decreased levels of pro-apoptotic markers. We assume that resistance of rat heart to I/R injury starts to already decline during maturation, and that RIPC may represent a clinically relevant cardioprotective intervention in the elder population.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2022
    Elektronická adresahttps://www.mdpi.com/1422-0067/22/20/11009
Počet záznamů: 1  

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