Počet záznamů: 1
Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64
- 1.
SYSNO ASEP 0544992 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64 Tvůrce(i) Kuzma, Marek (MBU-M) ORCID, RID
Hájek, Jan (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Gardiner, Alastair T. (MBU-M) ORCID
Lukeš, Martin (MBU-M) ORCID
Moos, Martin (BC-A) RID, ORCID
Šimek, Petr (BC-A) RID, ORCID
Koblížek, Michal (MBU-M) RID, ORCID
Nupur, Nupur (MBU-M) ORCIDČíslo článku 15964 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova energy-transfer ; biosynthetic-pathway ; ft-ir ; raman ; bacteriochlorophyll ; evolution ; strain ; synechoxanthin ; thiorhodaceae ; spectroscopy Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Biochemie CEP GX19-28778X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000684439300023 EID SCOPUS 85112619992 DOI 10.1038/s41598-021-95254-6 Anotace Gemmatimonas phototrophica AP64 is the first phototrophic representative of the bacterial phylum Gemmatimonadetes. The cells contain photosynthetic complexes with bacteriochlorophyll a as the main light-harvesting pigment and an unknown carotenoid with a single broad absorption band at 490 nm in methanol. The carotenoid was extracted from isolated photosynthetic complexes, and purified by liquid chromatography. A combination of nuclear magnetic resonance (H-1 NMR, COSY, H-1-C-13 HSQC, H-1-C-13 HMBC, J-resolved, and ROESY), high-resolution mass spectroscopy, Fourier-transformed infra-red, and Raman spectroscopy was used to determine its chemical structure. The novel linear carotenoid, that we have named gemmatoxanthin, contains 11 conjugated double bonds and is further substituted by methoxy, carboxyl and aldehyde groups. Its IUPAC-IUBMB semi-systematic name is 1 'Methoxy-19 'oxo-3 ' ,4 'didehydro-7,8,1 ' ,2 'tetrahydro- Psi, Psi carotene-16-oic acid. To our best knowledge, the presence of the carboxyl, methoxy and aldehyde groups on a linear C40 carotenoid backbone is reported here for the first time. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s41598-021-95254-6
Počet záznamů: 1