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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
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SYSNO ASEP 0539261 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results Tvůrce(i) Moller, P. (DK)
Azqueta, A. (ES)
Boutet-Robinet, E. (FR)
Koppen, G. (BE)
Bonassi, S. (IT)
Milić, M. (HR)
Gajski, G. (IT)
Costa, S. (PT)
Teixeira, J. P. (PT)
Costa Pereira, C. (PT)
Dusinska, M. (NO)
Godschalk, R. (NL)
Brunborg, G. (NO)
Gützkow, K.B. (NO)
Giovannelli, L. (IT)
Cooke, M.S. (US)
Richling, E. (DE)
Laffon, B. (ES)
Valdiglesias, V. (ES)
Basaran, N. (TR)
Del Bo', C. (IT)
Zegura, B. (SK)
Novák, M. (SK)
Stopper, H. (DE)
Vodička, Pavel (UEM-P) RID
Vodenková, Soňa (UEM-P) ORCID, RID
de Andrade, V.M. (BR)
Sramkova, M. (SK)
Gábelová, A. (SK)
Collins, A. (NO)
Langie, S.A.S. (NL)Zdroj.dok. Nature Protocols. - : Nature Publishing Group - ISSN 1754-2189
Roč. 15, č. 12 (2020), s. 3817-3826Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova inter-laboratory variation ; DNA-damage ; animal-models Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000583953500001 EID SCOPUS 85093963487 DOI 10.1038/s41596-020-0398-1 Anotace The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between desirable and essential information: essential information refers to the precise details that are necessary to assess the quality of the experimental work, whereas desirable information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41596-020-0398-1
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