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Translational Research in Serious Human Diseases
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SYSNO ASEP 0536629 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Laser microirradiation as a tool for DNA damage response studies Tvůrce(i) Vaškovičová, Michaela (UZFG-Y) ORCID
Šolc, Petr (UZFG-Y) RID, ORCIDZdroj.dok. Translational Research in Serious Human Diseases. - Praha : Academia, 2020 / Kello M. ; Strnadel J. ; Klempir J. ; Roth J. ; Myslivcová-Fučíková A. ; Hansíková H. ; Kozák I. - ISBN 978-80-200-3158-7 Rozsah stran s. 257-264 Poč.str. 8 s. Poč.str.knihy 271 Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova laser microirradiation ; DNA damage response ; double-stranded DNA breaks Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP LO1609 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UZFG-Y - RVO:67985904 Anotace Double-strand DNA breaks (DSBs) are considered as the most hazardous type of DNA lesions. Recognition of DSBs, temporal cell cycle arrest and activation of repair pathways are crucial steps in response to DNA damage. Errors in any of these steps can lead to adverse consequences affecting genome integrity, e.g. chromosomal aberrations or generation of mutations. The genomic instability caused by severe endpoints of DSBs processing can lead to cell death or development of cancer. Therefore, it is crucial to study all aspects of DNA damage response in cells.
In the context of studying DNA damage response in cells, DSBs are typically induced either by γ-irradiation or by UV light. DSBs can be also induced by radiomimetic drugs, such as Neocarzinostatin. However, these techniques have several limitations, e.g. inability to induced DSBs only in a specific region of nucleus. Therefore, the laser UV microirradiation technique combined with confocal microscopy was developed to study the DNA damage response in living cells.Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2021
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