Počet záznamů: 1
Substrate-dependent incorporation of carbon and hydrogen for lipid biosynthesis by Methanosarcina barkeri
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SYSNO ASEP 0536304 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Substrate-dependent incorporation of carbon and hydrogen for lipid biosynthesis by Methanosarcina barkeri Tvůrce(i) Wu, W. (DE)
Meador, Travis Blake (BC-A) ORCID, RID
Könneke, M. (DE)
Elvert, M. (DE)
Wegener, G. (DE)
Hinrichs, K.-U. (DE)Zdroj.dok. Environmental Microbiology Reports. - : Wiley - ISSN 1758-2229
Roč. 12, č. 5 (2020), s. 555-567Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova stable isotope probing ; carbon fixation ; methanogen ; anaplerotic Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LM2015075 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001782 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000571477600010 EID SCOPUS 85091199654 DOI 10.1111/1758-2229.12876 Anotace Dual stable isotope probing has been used to infer rates of microbial biomass production and modes of carbon fixation. In order to validate this approach for assessing archaeal production, the methanogenic archaeon Methanosarcina barkeri was grown either with H2, acetate or methanol with D2O and 13C-dissolved inorganic carbon (DIC). Our results revealed unexpectedly low D incorporation into lipids, with the net fraction of water-derived hydrogen amounting to 0.357 ± 0.042, 0.226 ± 0.003 and 0.393 ± 0.029 for growth on H2/CO2, acetate and methanol respectively. The variability in net water H assimilation into lipids during the growth of M. barkeri on different substrates is possibly attributed to different Gibbs free energy yields, such that higher energy yield promoted the exchange of hydrogen between medium water and lipids. Because NADPH likely serves as the portal for H transfer, increased NADPH production and/or turnover associated with high energy yield may explain the apparent differences in net water H assimilation into lipids. The variable DIC and water H incorporation into M. barkeri lipids imply systematic, metabolic patterns of isotope incorporation and suggest that the ratio of 13C-DIC versus D2O assimilation in environmental samples may serve as a proxy for microbial energetics in addition to microbial production and carbon assimilation pathways. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://sfamjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/1758-2229.12876
Počet záznamů: 1