Počet záznamů: 1
Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
- 1.0533050 - MBÚ 2021 RIV GB eng J - Článek v odborném periodiku
Sushytskyi, L. - Lukáč, Pavol - Synytsya, A. - Bleha, R. - Rajsiglová, Lenka - Capek, P. - Pohl, Radek - Vannucci, Luca - Čopiková, J. - Kaštánek, P.
Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae).
Carbohydrate Polymers. Roč. 246, OCT 15 (2020), č. článku 116588. ISSN 0144-8617. E-ISSN 1879-1344
Institucionální podpora: RVO:61388971 ; RVO:61388963
Klíčová slova: cell-wall polysaccharide * infrared-spectroscopy * immunostimulatory polysaccharides * sulfated polysaccharides * lutein production * molecular-weight * galactofuranose * Xylogalactofuranan
Obor OECD: Microbiology; Biochemistry and molecular biology (UOCHB-X)
Impakt faktor: 9.381, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0144861720307621
Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
Trvalý link: http://hdl.handle.net/11104/0311551
Počet záznamů: 1