Počet záznamů: 1
Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
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SYSNO ASEP 0532865 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema Tvůrce(i) Zvanarou, S. (CN)
Vágnerová, Radka (UEB-Q) ORCID
Mackievic, V. (BY)
Usnich, S. (BY)
Smolich, I. (BY)
Sokolik, A. (BY)
Yu, M. (CN)
Huang, X. (CN)
Angelis, Karel (UEB-Q) RID, ORCID
Demidchik, V. (BY)Celkový počet autorů 10 Číslo článku 104236 Zdroj.dok. Environmental and Experimental Botany. - : Elsevier - ISSN 0098-8472
Roč. 180, December (2020)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Abiotic stress ; Antioxidants ; Comet assay ; Double-strand DNA breaks ; Hydroxyl radicals ; Physcomitrella patens ; Protonema ; Reactive oxygen species ; Salinity ; Single-strand DNA breaks Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP LTC17047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000579447700008 EID SCOPUS 85090573884 DOI 10.1016/j.envexpbot.2020.104236 Anotace One of the early reactions of plants to high levels of NaCl is the generation of reactive oxygen species (ROS), which is critical for stress signaling, Na+ extrusion, oxidative damage and programmed cell death (PCD) under salt stress. Using moss Physcomitrella patens protonema as a model system, we have tested whether ROS generation and DNA degradation can be induced by salinity in Bryophyta. We have shown with dihydroethidium in vivo test that toxic NaCl concentrations significantly stimulate ROS production. The pharmacological analysis carried out using superoxide dismutase, catalase, dimethyl sulfoxide, thiourea and spermine outlines the superoxide and hydroxyl radicals as major NaCl-induced species. Using Comet assay, we have found that NaCl affects the DNA stability in ROS-dependent manner, increasing both single and double strand DNA breaks (SSBs and DSBs, respectively). Surprisingly, DSBs were induced by NaCl to a lesser extent than SSBs. This is indicative of an unexpected and sophisticated action of NaCl on moss genome including DSBs through PCD and SSBs caused by hydroxyl radical-induced DNA oxidation or transposon rearrangement. We also report a relatively high sensitivity of Physcomitrella patens protonema to NaCl compared to later developmental stages of this moss, suggesting that dividing cells are more sensitive to NaCl than other tissues. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.1016/j.envexpbot.2020.104236
Počet záznamů: 1