Počet záznamů: 1  

Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema

  1. 1.
    SYSNO ASEP0532865
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevSalt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
    Tvůrce(i) Zvanarou, S. (CN)
    Vágnerová, Radka (UEB-Q) ORCID
    Mackievic, V. (BY)
    Usnich, S. (BY)
    Smolich, I. (BY)
    Sokolik, A. (BY)
    Yu, M. (CN)
    Huang, X. (CN)
    Angelis, Karel (UEB-Q) RID, ORCID
    Demidchik, V. (BY)
    Celkový počet autorů10
    Číslo článku104236
    Zdroj.dok.Environmental and Experimental Botany. - : Elsevier - ISSN 0098-8472
    Roč. 180, December (2020)
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaAbiotic stress ; Antioxidants ; Comet assay ; Double-strand DNA breaks ; Hydroxyl radicals ; Physcomitrella patens ; Protonema ; Reactive oxygen species ; Salinity ; Single-strand DNA breaks
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDBiochemistry and molecular biology
    CEPLTC17047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaUEB-Q - RVO:61389030
    UT WOS000579447700008
    EID SCOPUS85090573884
    DOI10.1016/j.envexpbot.2020.104236
    AnotaceOne of the early reactions of plants to high levels of NaCl is the generation of reactive oxygen species (ROS), which is critical for stress signaling, Na+ extrusion, oxidative damage and programmed cell death (PCD) under salt stress. Using moss Physcomitrella patens protonema as a model system, we have tested whether ROS generation and DNA degradation can be induced by salinity in Bryophyta. We have shown with dihydroethidium in vivo test that toxic NaCl concentrations significantly stimulate ROS production. The pharmacological analysis carried out using superoxide dismutase, catalase, dimethyl sulfoxide, thiourea and spermine outlines the superoxide and hydroxyl radicals as major NaCl-induced species. Using Comet assay, we have found that NaCl affects the DNA stability in ROS-dependent manner, increasing both single and double strand DNA breaks (SSBs and DSBs, respectively). Surprisingly, DSBs were induced by NaCl to a lesser extent than SSBs. This is indicative of an unexpected and sophisticated action of NaCl on moss genome including DSBs through PCD and SSBs caused by hydroxyl radical-induced DNA oxidation or transposon rearrangement. We also report a relatively high sensitivity of Physcomitrella patens protonema to NaCl compared to later developmental stages of this moss, suggesting that dividing cells are more sensitive to NaCl than other tissues.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2021
    Elektronická adresahttp://doi.org/10.1016/j.envexpbot.2020.104236
Počet záznamů: 1  

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