Počet záznamů: 1
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
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SYSNO ASEP 0511603 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria Tvůrce(i) Farka, Z. (CZ)
Mickert, M. J. (DE)
Hlaváček, Antonín (UIACH-O) ORCID
Poláchová, V. (CZ)
Kostiv, Uliana (UMCH-V) RID
Pastucha, M. (CZ)
Horák, Daniel (UMCH-V) RID, ORCID
Gorris, H. H. (DE)
Skládal, P. (CZ)Celkový počet autorů 9 Zdroj.dok. NANOCON Conference Proceedings - International Conference on Nanomaterials, Volume 2020-October. - Ostrava : Tanger Ltd., 2020 - ISSN 2694-930X - ISBN 978-808729495-6 Rozsah stran s. 311-316 Poč.str. 6 s. Forma vydání Online - E Akce NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./ Datum konání 16.10.2019 - 18.10.2019 Místo konání Brno Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova single-molecule detection ; photon-upconversion nanoparticle ; bioconjugation Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP LTAB19011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UIACH-O - RVO:68081715 ; UMCH-V - RVO:61389013 UT WOS 000664115400051 EID SCOPUS 85097197157 DOI 10.37904/nanocon.2019.8445 Anotace The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2021 Elektronická adresa https://www.confer.cz/nanocon/2019
Počet záznamů: 1