Počet záznamů: 1
MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
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SYSNO ASEP 0509780 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells Tvůrce(i) Štefková, K. (CZ)
Hanackova, M. (CZ)
Kučera, J. (CZ)
Radaszkiewicz, K. A. (CZ)
Ambrůzová, Barbora (BFU-R)
Kubala, Lukáš (BFU-R) RID, ORCID
Pacherník, J. (CZ)Celkový počet autorů 7 Číslo článku 5128135 Zdroj.dok. Stem Cells International - ISSN 1687-966X
Roč. 2019, Č. 2019 (2019)Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova c/ebp Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Cell biology Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000472008000001 DOI 10.1155/2019/5128135 Anotace The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2020 Elektronická adresa http://downloads.hindawi.com/journals/sci/2019/5128135.pdf
Počet záznamů: 1