Carbohydrate-naphthalene diimide conjugates as potential antiparasitic drugs: Synthesis, evaluation and structure-activity studies

0502860 - BFÚ 2019 RIV FR eng J - Článek v odborném periodiku
Zuffo, M. - Stucchi, M. - Campos-Salinas, J. - Cabello-Donayre, M. - Martínez-García, M. - Belmonte-Reche, E. - Perez-Victoria, J. M. - Mergny, Jean-Louis - Freccero, M. - Morales, J. C. - Doria, F.
Carbohydrate-naphthalene diimide conjugates as potential antiparasitic drugs: Synthesis, evaluation and structure-activity studies.
European Journal of Medicinal Chemistry. Roč. 163, FEB 1 2019 (2019), s. 54-66. ISSN 0223-5234. E-ISSN 1768-3254
Grant CEP: GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: g-quadruplex ligands * rna g-quadruplexes * dna * design
Obor OECD: Pharmacology and pharmacy
Impakt faktor: 5.573, rok: 2019
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0223523418310031?via%3Dihub

The neglected tropical diseases Human African Trypanosomiasis and leishmaniasis are caused by infection with trypanosomatid parasites Trypanosoma brucei and Leishmania spp, respectively. The genomes of these organisms contain multiple putative G-quadruplex (G4) forming sequences which have recently been proposed to mediate processes relevant for parasite survival. Therefore, G4 could be considered as potential targets for a novel approach towards the development of antiparasitic drugs. Recently, we have demonstrated that G4 ligands such as carbohydrate naphthalene diimide conjugates (carb-NDIs) possess notable antiparasitic activity. Herein, we have synthesized a new family of carb-NDIs, characterized by significant structural variability, and evaluated their anti-parasitic activity, with special focus on T brucei. The interaction with relevant G4 sequences was evaluated in vitro through independent biophysical methods (FRET melting assays under competing conditions with double stranded DNA, circular dichroism and fluorescence titrations). Finally, flow cytometry and confocal microscopy experiments demonstrated that the conjugates exhibit excellent uptake into T. brucei parasites, localizing in the nuclei and kinetoplasts. Promising antiparasitic activity and selectivity against control mammalian cells, together with their peculiar mechanism of action, render the carb-NDI conjugates as suitable candidates for the development of an innovative treatment of trypanosomiasis. (C) 2018 Elsevier Masson SAS. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0294739