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The NA(v)1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing
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SYSNO ASEP 0489199 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The NA(v)1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing Tvůrce(i) Torres-Pérez, J. V. (GB)
Adámek, Pavel (FGU-C) RID, ORCID, SAI
Paleček, Jiří (FGU-C) RID, ORCID
Vizcaychipi, M. (GB)
Nagy, I. (GB)
Varga, A. (HU)Zdroj.dok. Journal of Molecular Medicine-Jmm. - : Springer - ISSN 0946-2716
Roč. 96, č. 1 (2018), s. 75-84Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova pain ; p-ERK1/2 ; primary sensory neuron ; p-S10H3 ; spinal cord Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA15-11138S GA ČR - Grantová agentura ČR LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LH15279 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000419131300008 EID SCOPUS 85031925522 DOI 10.1007/s00109-017-1599-0 Anotace Controlling pain in burn-injured patients poses a major clinical challenge. Recent findings suggest that reducing the activity of the voltage-gated sodium channel Na(v)1.7 in primary sensory neurons could provide improved pain control in burn-injured patients. Here, we report that partial thickness scalding-type burn injury on the rat paw upregulates Na(v)1.7 expression in primary sensory neurons 3 h following injury. The injury also induces upregulation in phosphorylated cyclic adenosine monophosphate response element-binding protein (p-CREB), a marker for nociceptive activation in primary sensory neurons. The upregulation in p-CREB occurs mainly in Na(v)1.7-immunopositive neurons and exhibits a peak at 5 min and, following a decline at 30 min, a gradual increase from 1 h post-injury. The Na(v)1.7 blocker protoxin II (ProTxII) or morphine injected intraperitoneally 15 min before or after the injury significantly reduces burn injury-induced spinal upregulation in phosphorylated serine 10 in histone H3 and phosphorylated extracellular signal-regulated kinase 1/2, which are both markers for spinal nociceptive processing. Further, ProTxII significantly reduces the frequency of spontaneous excitatory post-synaptic currents in spinal dorsal horn neurons following burn injury. Together, these findings indicate that using Na(v)1.7 blockers should be considered to control pain in burn injury. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
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