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Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome
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SYSNO ASEP 0489197 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome Tvůrce(i) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Pataridis, Statis (FGU-C)
Eckhardt, Adam (FGU-C) RID, ORCID
Sedmera, David (FGU-C) RID, ORCID, SAIZdroj.dok. Protein Journal. - : Springer - ISSN 1572-3887
Roč. 37, č. 1 (2018), s. 21-37Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova proteins of eggshell ; eggshell pigment ; crocodile Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA15-01948S GA ČR - Grantová agentura ČR GA16-02972S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000426949100004 EID SCOPUS 85034666012 DOI 10.1007/s10930-017-9750-x Anotace The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs). Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
Počet záznamů: 1