Počet záznamů: 1
Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids)
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SYSNO ASEP 0483972 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids) Tvůrce(i) Sedlačík, Tomáš (UMCH-V) RID
Acar, O. K. (TR)
Studenovská, Hana (UMCH-V) RID, ORCID
Kotelnikov, Ilya (UMCH-V) RID, ORCID
Kučka, Jan (UMCH-V) RID, ORCID
Konečná, Z. (CZ)
Zikmund, T. (CZ)
Kaiser, J. (CZ)
Köse, G. T. (TR)
Rypáček, František (UMCH-V) RIDZdroj.dok. Soft Matter - ISSN 1744-683X
Roč. 14, č. 2 (2018), s. 228-238Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova poly(amino acids) ; cryogelation ; scaffold Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000419352600006 EID SCOPUS 85040250837 DOI 10.1039/C7SM02074K Anotace In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide–alkyne “click”-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2019
Počet záznamů: 1