Počet záznamů: 1
Assessment of the synbiotic properites of human milk oligosaccharides and Bifidobacterium longum subsp infantis in vitro and in humanised mice
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SYSNO ASEP 0475173 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Assessment of the synbiotic properites of human milk oligosaccharides and Bifidobacterium longum subsp infantis in vitro and in humanised mice Tvůrce(i) Musilová, Š. (CZ)
Modráčková, N. (CZ)
Hermanová, Petra (MBU-M) ORCID
Hudcovic, Tomáš (MBU-M) RID, ORCID
Švejstil, R. (CZ)
Rada, V. (CZ)
Tejnecký, V. (CZ)
Bunešová, V. (CZ)Zdroj.dok. Beneficial Microbes - ISSN 1876-2883
Roč. 8, č. 2 (2017), s. 281-289Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova human milk ; bifidobacteria ; synbiotics Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA15-07268S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000400167600013 EID SCOPUS 85018245854 DOI 10.3920/BM2016.0138 Anotace he mode of delivery plays a crucial role in infant gastrointestinal tract colonisation, which in the case of caesarean section is characterised by the presence of clostridia and low bifidobacterial counts. Gut colonisation can be modified by probiotics, prebiotics or synbiotics. Human milk oligosaccharides (HMOs) are infant prebiotics that show a bifidogenic effect. Moreover, genome sequencing of Bifidobacterium longum subsp. infantis within the infant microbiome revealed adaptations for milk utilisation. This study aimed to evaluate the synbiotic effect of B. longum subsp. infantis, HMOs and human milk (HM) both in vitro and in vivo (in a humanised mouse model) in the presence of faecal microbiota from infants born by caesarean section. The combination of B. longum and HMOs or HM reduced the clostridia and G-bacteria counts both in vitro and in vivo. The bifidobacterial population in vitro significantly increased and produce high concentrations of acetate and lactate. In vitro competition assays confirmed that the tested bifidobacterial strain is a potential probiotic for infants and, together with HMOs or HM, acts as a synbiotic. It is also able to inhibit potentially pathogenic bacteria. The synbiotic effects identified in vitro were not observed in vivo. However, there was a significant reduction in clostridia counts in both experimental animal groups (HMOs + B. longum and HM + B. longum), and a specific immune response via increased interleukin (IL)10 and IL-6 production. Animal models do not perfectly mimic human conditions, however, they are essential for testing the safety of functional foods. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1