Počet záznamů: 1
Transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of Bordetella adenylate cyclase toxin
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SYSNO ASEP 0460039 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of Bordetella adenylate cyclase toxin Tvůrce(i) Wald, Tomáš (MBU-M) RID
Osičková, Adriana (MBU-M) RID, ORCID
Mašín, Jiří (MBU-M) RID, ORCID
Matyska Lišková, Petra (MBU-M)
Petry-Podgórska, Inga (UIACH-O)
Matoušek, Tomáš (UIACH-O) RID, ORCID
Šebo, Peter (MBU-M) RID, ORCID
Osička, Radim (MBU-M) RID, ORCIDZdroj.dok. Pathogens and Disease - ISSN 2049-632X
Roč. 74, č. 3 (2016), flw008Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova adenylate cyclase toxin ; ICP-MS ; CD11b/CD18 Vědní obor RIV EE - Mikrobiologie, virologie Vědní obor RIV – spolupráce Ústav analytické chemie - Analytická chemie, separace CEP GAP302/11/0580 GA ČR - Grantová agentura ČR GAP302/12/0460 GA ČR - Grantová agentura ČR GA13-14547S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 ; UIACH-O - RVO:68081715 UT WOS 000374475700012 DOI 10.1093/femspd/ftw008 Anotace Adenylate cyclase toxin-hemolysin (CyaA, ACT or AC-Hly) of the whooping cough agent Bordetella pertussis penetrates phagocytes expressing the integrin complement receptor 3 (CR3, CD11b/CD18, alpha(M)beta(2) or Mac-1). CyaA translocates its adenylate cyclase (AC) enzyme domain into cell cytosol and catalyzes unregulated conversion of ATP to cAMP, thereby subverting cellular signaling. In parallel, CyaA forms small cation-selective membrane pores that permeabilize cells for potassium efflux, contributing to cytotoxicity of CyaA and eventually provoking colloid-osmotic cell lysis. To investigate whether the single-pass alpha-helical transmembrane segments of CR3 subunits CD11b and CD18 do directly participate in AC domain translocation and/or pore formation by the toxin, we expressed in CHO cells variants of CR3 that contained artificial transmembrane segments, or lacked the transmembrane segment(s) at all. The results demonstrate that the transmembrane segments of CR3 are not directly involved in the cytotoxic activities of CyaA but serve for maintaining CR3 in a conformation that is required for efficient toxin binding and action. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1