Počet záznamů: 1
Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment
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SYSNO ASEP 0459242 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment Tvůrce(i) Hu, XQ. (CN)
Cifarelli, V. (US)
Sun, SS. (CN)
Kuda, Ondřej (FGU-C) RID, ORCID, SAI
Abumrad, N. A. (US)
Su, X. (CN)Zdroj.dok. Journal of Lipid Research. - : Elsevier - ISSN 0022-2275
Roč. 57, č. 4 (2016), s. 663-673Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova adipose tissue ; cyclooxygenase ; eicosanoids ; extracellular signal-regulated kinase ; fatty acid ; inflammation ; lipase Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa CEP LH14040 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000373924600015 EID SCOPUS 84963864745 DOI 10.1194/jlr.M066530 Anotace Obesity induces accumulation of adipose tissue macrophages (ATMs), which contribute to both local and systemic inflammation and modulate insulin sensitivity. Adipocyte lipolysis during fasting and weight loss also leads to ATM accumulation, but without proinflammatory activation suggesting distinct mechanisms of ATM recruitment. We examined the possibility that specific lipid mediators with anti-inflammatory properties are released from adipocytes undergoing lipolysis to induce macrophage migration. In the present study, we showed that conditioned medium (CM) from adipocytes treated with forskolin to stimulate lipolysis can induce migration of RAW 264.7 macrophages. In addition to FFAs, lipolytic stimulation increased release of prostaglandin E2 (PGE2) and prostaglandin D2 (PGD2), reflecting cytosolic phospholipase A2 α activation and enhanced cyclooxygenase (COX) 2 expression. Reconstituted medium with the anti-inflammatory PGE2 potently induced macrophage migration while different FFAs and PGD2 had modest effects. The ability of CM to induce macrophage migration was abolished by treating adipocytes with the COX2 inhibitor sc236 or by treating macrophages with the prostaglandin E receptor 4 antagonist AH23848. In fasted mice, macrophage accumulation in adipose tissue coincided with increases of PGE2 levels and COX1 expression. Collectively, our data show that adipocyte-originated PGE2 with inflammation suppressive properties plays a significant role in mediating ATM accumulation during lipolysis. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
Počet záznamů: 1