Počet záznamů: 1  

Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment

  1. 1.
    SYSNO ASEP0459242
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMajor role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment
    Tvůrce(i) Hu, XQ. (CN)
    Cifarelli, V. (US)
    Sun, SS. (CN)
    Kuda, Ondřej (FGU-C) RID, ORCID, SAI
    Abumrad, N. A. (US)
    Su, X. (CN)
    Zdroj.dok.Journal of Lipid Research. - : Elsevier - ISSN 0022-2275
    Roč. 57, č. 4 (2016), s. 663-673
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaadipose tissue ; cyclooxygenase ; eicosanoids ; extracellular signal-regulated kinase ; fatty acid ; inflammation ; lipase
    Vědní obor RIVFB - Endokrinologie, diabetologie, metabolizmus, výživa
    CEPLH14040 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000373924600015
    EID SCOPUS84963864745
    DOI10.1194/jlr.M066530
    AnotaceObesity induces accumulation of adipose tissue macrophages (ATMs), which contribute to both local and systemic inflammation and modulate insulin sensitivity. Adipocyte lipolysis during fasting and weight loss also leads to ATM accumulation, but without proinflammatory activation suggesting distinct mechanisms of ATM recruitment. We examined the possibility that specific lipid mediators with anti-inflammatory properties are released from adipocytes undergoing lipolysis to induce macrophage migration. In the present study, we showed that conditioned medium (CM) from adipocytes treated with forskolin to stimulate lipolysis can induce migration of RAW 264.7 macrophages. In addition to FFAs, lipolytic stimulation increased release of prostaglandin E2 (PGE2) and prostaglandin D2 (PGD2), reflecting cytosolic phospholipase A2 α activation and enhanced cyclooxygenase (COX) 2 expression. Reconstituted medium with the anti-inflammatory PGE2 potently induced macrophage migration while different FFAs and PGD2 had modest effects. The ability of CM to induce macrophage migration was abolished by treating adipocytes with the COX2 inhibitor sc236 or by treating macrophages with the prostaglandin E receptor 4 antagonist AH23848. In fasted mice, macrophage accumulation in adipose tissue coincided with increases of PGE2 levels and COX1 expression. Collectively, our data show that adipocyte-originated PGE2 with inflammation suppressive properties plays a significant role in mediating ATM accumulation during lipolysis.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2017
Počet záznamů: 1  

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