Počet záznamů: 1
The Arabidopsis mitogen-activated protein kinase 6 is associated with γ-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress
- 1.
SYSNO ASEP 0447446 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Arabidopsis mitogen-activated protein kinase 6 is associated with γ-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress Tvůrce(i) Kohoutová, Lucie (MBU-M) RID
Kourová, Hana (MBU-M)
Nagy, S. K. (HU)
Volc, Jindřich (MBU-M) RID
Halada, Petr (MBU-M) RID, ORCID
Mészáros, T. (HU)
Meskiene, I. (AT)
Bögre, L. (GB)
Binarová, Pavla (MBU-M) RID, ORCIDZdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 207, č. 4 (2015), s. 1061-1074Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis ; cell division ; EB1c Vědní obor RIV EE - Mikrobiologie, virologie CEP 7AMB13AT013 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP501/12/2333 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000358965800016 DOI 10.1111/nph.13501 Anotace Stress-activated plant mitogen-activated protein (MAP) kinase pathways play roles in growth adaptation to the environment by modulating cell division through cytoskeletal regulation, but the mechanisms are poorly understood. We performed protein interaction and phosphorylation experiments with cytoskeletal proteins, mass spectrometric identification of MPK6 complexes and immunofluorescence analyses of the microtubular cytoskeleton of mitotic cells using wild-type, mpk6-2 mutant and plants overexpressing the MAP kinase-inactivating phosphatase, AP2C3. We showed that MPK6 interacted with -tubulin and co-sedimented with plant microtubules polymerized invitro. It was the active form of MAP kinase that was enriched with microtubules and followed similar dynamics to -tubulin, moving from poles to midzone during the anaphase-to-telophase transition. We found a novel substrate for MPK6, the microtubule plus end protein, EB1c. The mpk6-2 mutant was sensitive to 3-nitro-l-tyrosine (NO2-Tyr) treatment with respect to mitotic abnormalities, and root cells overexpressing AP2C3 showed defects in chromosome segregation and spindle orientation. Our data suggest that the active form of MAP kinase interacts with -tubulin on specific subsets of mitotic microtubules during late mitosis. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2016
Počet záznamů: 1