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Prdm9 incompatibility controls oligospermia and delayed fertility but no selfish transmission in mouse intersubspecific hybrids
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SYSNO ASEP 0428332 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Prdm9 incompatibility controls oligospermia and delayed fertility but no selfish transmission in mouse intersubspecific hybrids Tvůrce(i) Flachs, Petr (UMG-J)
Bhattacharyya, Tanmoy (UMG-J)
Mihola, Ondřej (UMG-J) RID, ORCID
Piálek, Jaroslav (UBO-W) RID, ORCID, SAI
Forejt, Jiří (UMG-J) RID, ORCID
Trachtulec, Zdeněk (UMG-J) RID, ORCIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 9, č. 4 (2014), e95806Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova hybrid sterility ; meiosis ; segregation distortion Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/10/1931 GA ČR - Grantová agentura ČR GA206/08/0640 GA ČR - Grantová agentura ČR GPP305/11/P630 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 ; UBO-W - RVO:68081766 UT WOS 000335240300098 DOI 10.1371/journal.pone.0095806 Anotace PR-domain 9 (Prdm9) is the first hybrid sterility gene identified in mammals. The incompatibility between Prdm9 from Mus musculus domesticus (Mmd; the B6 strain) and the Hstx2 region of chromosome (Chr) X from M. m. musculus (Mmm; the PWD strain) participates in the complete meiotic arrest of mouse intersubspecific (PWD×B6)F1 hybrid males. Other studies suggest that also semisterile intersubspecific hybrids are relevant for mouse speciation, but the genes responsible remain unknown. To investigate the causes of this semisterility, we analyzed the role of Prdm9 and Chr X in hybrids resulting from the crosses of PWK, another Mmm-derived inbred strain. We demonstrate that Prdm9 and Chr X control the partial meiotic arrest and reduced sperm count in (PWK×B6)F1 males. Asynapsis of heterosubspecific chromosomes and semisterility were partially suppressed by removal of the B6 allele of Prdm9. Polymorphisms between PWK and PWD on Chr X but not in the Prdm9 region were responsible for the modification of the outcome of Prdm9 - Chr X F1 hybrid incompatibility. Furthermore, (PWK×B6)F1 hybrid males displayed delayed fertility dependent on the Prdm9 incompatibility. While the Drosophila hybrid sterility gene Overdrive causes both delayed fertility and increased transmission of its own chromosome to the offspring, the segregation of Chr X and the Prdm9 region from the mouse (PWK×B6)F1 males was normal. Our results indicate extended functional consequences of Prdm9 - Chr X intersubspecific incompatibility on the fertility of hybrids and should influence the design of fertility analyses in hybrid zones and of laboratory crosses between Mmm and Mmd strains. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2014
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