Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides
1.
SYSNO ASEP
0370593
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
Záznam nebyl označen do RIV
Název
Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides
Tvůrce(i)
Neubauerová, Tereza (UOCHB-X) Macková, Martina (UOCHB-X) Macek, Tomáš (UOCHB-X) Šanda, Miloslav (UOCHB-X) Voburka, Zdeněk (UOCHB-X)
Celkový počet autorů
5
Zdroj.dok.
Biologically Active Peptides. 12th Conference. - Praha : Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 / Slaninová J.
- ISBN 978-80-86241-44-9
The antibiotic resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our laboratory, several peptide fractions with antimicrobial acivity against gram-positive and gramnegative bacteria and fungi were isolated from larvae of flesh fly Neobellieria bullata. The presence of peptides was proved by tricine electrophoresis, molecular weights and sequences determination by MS and Edman sequencing, respectively. In comparison with database sequences we obtained structures present mainly in insect and amphibian antimicrobial peptides, but total matches were not higher than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Antimicrobial activity of these peptides was determined.
