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Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells
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SYSNO ASEP 0349924 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells Tvůrce(i) Pejchar, Přemysl (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCID
Novotná, Z. (CZ)
Veselková, Štěpánka (UEB-Q)
Kocourková, Daniela (UEB-Q) ORCID
Valentová, O. (CZ)
Schwarzerová, K. (CZ)
Martinec, Jan (UEB-Q) RID, ORCIDZdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 188, č. 1 (2010), s. 150-160Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova aluminium (AL) ; BY-2 ; diacylglycerol (DAG) Vědní obor RIV ED - Fyziologie CEP GA522/07/1614 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000281551500017 DOI 10.1111/j.1469-8137.2010.03349.x Anotace Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family.We labelled the tobacco cell line BY-2 and pollen tubes with a fluorescent derivative of phosphatidylcholine and assayed for patterns of fluorescently labelled products. Growth of pollen tubes was analysed.We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl3. Investigation of possible metabolic pathways that control DAG generation and consumption during the response to Al showed that DAG originated from the reaction catalysed by PC-PLC. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line. The growth of pollen tubes arrested by Al was rescued by externally added DAG. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2011
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