Počet záznamů: 1
RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening
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SYSNO ASEP 0349491 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening Tvůrce(i) Štefanič, S. (US)
Dvořák, Jan (BC-A)
Horn, Martin (UOCHB-X) RID, ORCID
Braschi, S. (US)
Sojka, Daniel (BC-A) RID, ORCID
Ruelas, D. (US)
Suzuki, B. (US)
Lim, K.-C. (US)
Hopkins, S. D. (US)
McKerrow, J. H. (US)
Caffrey, C. R. (US)Zdroj.dok. PLoS Neglected Tropical Diseases. - : Public Library of Science - ISSN 1935-2735
Roč. 4, č. 10 (2010), e850Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Tropical disease ; Schistosoma ; schistosomula ; RNAi ; protease ; tegument ; drug target ; drug discovery Vědní obor RIV EB - Genetika a molekulární biologie CEP GA203/09/1585 GA ČR - Grantová agentura ČR KJB600960911 GA AV ČR - Akademie věd LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ME10011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40550506 - UOCHB-X (2005-2011) AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000283559600019 DOI 10.1371/journal.pntd.0000850 Anotace RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1