Počet záznamů: 1
A fast and simple dot-immunobinding assay for quantifiction of mouse immunoglobulins in hybridoma culture supernatants
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SYSNO ASEP 0105288 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název A fast and simple dot-immunobinding assay for quantifiction of mouse immunoglobulins in hybridoma culture supernatants Překlad názvu Rychlý a jednoduchý imunovazebný test ("dot-immunobinding assay") pro kvantifikaci myších imunoglobulinů v supernatantech hybridomových kultur Tvůrce(i) Sulimenko, Tetyana (UMG-J)
Dráber, Pavel (UMG-J) RID, ORCIDZdroj.dok. Journal of Immunological Methods. - : Elsevier - ISSN 0022-1759
Roč. 289, - (2004), s. 89-95Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova dot-immunobinding assay ; hybridoma culture superntatants ; mouse immunoglobulins Vědní obor RIV EB - Genetika a molekulární biologie CEP IBS5052301 GA AV ČR - Akademie věd LN00A026 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 1P04OE158 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Anotace Mouse monoclonal antibodies of IgG subclasses and IgM class in hybridoma culture supernatants were quantified using a dot-immunobinding assay. Immunoglobulins were bound to nitrocellulose (NC) membrane and, after blocking, the membrane was incubated with anti-mouse antibody conjugated to horseradish peroxidase (HRP). Binding was revealed by incubation with a sensitive chemiluminiscence reagent. Quantitation was achieved by densitometric comparison with standard curves produced by purified monoclonal antibodies of the same subclass or purified antibodies of the same clone as the antibody to be quantified. These quantitative results were compared with those obtained using purified IgG from mouse serum or purified mouse myeloma IgM as standards. The dot-immunobinding assay requires 1 l of hybridoma culture sample and takes about 1 h in total. Good linearity between the staining intensity and the amount of immobilized immunoglobulins was observed over the range of 0.05ů5 ng/spot. The assay is simple, reproducible and can process simultaneously a large number of samples Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2005
Počet záznamů: 1