Počet záznamů: 1
Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus
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SYSNO ASEP 0099891 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus Překlad názvu Isoenzymy chitinas indukované TYMV a Leptosphaeria maculans při kompatibilní a inkompatibilní interakci u Brassica napus Tvůrce(i) Profotová, Bronislava (UEB-Q)
Burketová, Lenka (UEB-Q) RID, ORCID
Valentová, O. (CZ)Zdroj.dok. Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 51, č. 3 (2007), s. 507-513Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Chitinase ; TYMV ; Leptosphaeria maculans ; Brassica napus ; PR-proteins Vědní obor RIV GF - Choroby, škůdci, plevely a ochrana rostlin CEP GA522/03/0353 GA ČR - Grantová agentura ČR Další zdroj jiné veřejné zdroje CEZ AV0Z50380511 - UEB-Q (2005-2011) DOI 10.1007/s10535-007-0107-8 Anotace Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded to L. maculans infection. Chitinases started to accumulate before symptom appearance. First two acidic isozymes were detected 24 h after inoculation. The difference between compatible and incompatibe interaction reflected two basic isozymes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2008
Počet záznamů: 1