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Analysis of glycosylated cardiolipins from thermophilic bacteria using GC-MS and LC-ESI-MS/MS methods
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SYSNO ASEP 0584096 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of glycosylated cardiolipins from thermophilic bacteria using GC-MS and LC-ESI-MS/MS methods Tvůrce(i) Kyselová, L. (CZ)
Řezanka, Tomáš (MBU-M) ORCIDČíslo článku 115800 Zdroj.dok. Journal of Pharmaceutical and Biomedical Analysis. - : Elsevier - ISSN 0731-7085
Roč. 238, January 20 (2024)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova liquid-chromatography ; electrospray-ionization ; mass-spectrometry ; polar lipids ; identification ; methylation ; acids ; Thermophilic bacteria ; Partially methylated alditol acetates ; Glucosyl-cardiolipins ; Hydrophilic interaction liquid chromatography ; Electrospray mass spectrometry Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 001167197000001 EID SCOPUS 85174452321 DOI 10.1016/j.jpba.2023.115800 Anotace Unusual glucosesubstituted cardiolipins (Glcx-CLs) in three genera of thermophilic bacteria, having more than one glycosidically linked glucose to the hydroxyl of the central glycerol of Glcx-CLs were identified for the first time in thermophilic bacteria of the genera Geobacillus, Meiothermus, and Thermus. The number of glucoses reached up to five units. The structure of glycosidically linked oligosaccharides was determined based on shotgun analysis MS (electrospray highresolution tandem mass spectrometry), partially methylated alditol acetates were identified by GC-MS, both electron ionization (EI) and positive chemical ionization (PCI), hydrophilic interaction liquid chromatography (HILIC) separation and identification of CLs glycosides by high resolution MS-ESI, and digestion by specific glycosidases. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0731708523005691?via%3Dihub
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