Počet záznamů: 1
Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2
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SYSNO ASEP 0582327 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2 Tvůrce(i) Sulimenko, Vadym (UMG-J) RID, ORCID
Sládková, Vladimíra (UMG-J)
Sulimenko, Tetyana (UMG-J)
Dráberová, Eduarda (UMG-J) RID, ORCID
Vosecká, Věra (UMG-J)
Dráberová, Lubica (UMG-J) RID
Skalli, O. (US)
Dráber, Pavel (UMG-J) RID, ORCIDČíslo článku 1321321 Zdroj.dok. Frontiers in Immunology. - : Frontiers Media - ISSN 1664-3224
Roč. 15, Feb (2024)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova mast cells ; G protein-coupled receptor kinase-interacting protein 2 (GIT2) ; microtubule nucleation ; protein kinase C (PKC) ; centrosome Obor OECD Immunology CEP GA21-30281S GA ČR - Grantová agentura ČR GA23-07736S GA ČR - Grantová agentura ČR LTAUSA19118 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LUC23123 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 DOI https://doi.org/10.3389/fimmu.2024.1321321 Anotace Aggregation of high-affinity IgE receptors (FcϵRIs) on granulated mast cells triggers signaling pathways leading to a calcium response and release of inflammatory mediators from secretory granules. While microtubules play a role in the degranulation process, the complex molecular mechanisms regulating microtubule remodeling in activated mast cells are only partially understood. Here, we demonstrate that the activation of bone marrow mast cells induced by FcϵRI aggregation increases centrosomal microtubule nucleation, with G protein-coupled receptor kinase-interacting protein 2 (GIT2) playing a vital role in this process. Both endogenous and exogenous GIT2 were associated with centrosomes and g-tubulin complex proteins. Depletion of GIT2 enhanced centrosomal microtubule nucleation, and phenotypic rescue experiments revealed that GIT2, unlike GIT1, acts as a negative regulator of microtubule nucleation in mast cells. GIT2 also participated in the regulation of antigen-induced degranulation and chemotaxis. Further experiments showed that phosphorylation affected the centrosomal localization of GIT2 and that during antigen-induced activation, GIT2 was phosphorylated by conventional protein kinase C, which promoted microtubule nucleation. We propose that GIT2 is a novel regulator of microtubule organization in activated mast cells by modulating centrosomal microtubule nucleation. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2025 Elektronická adresa https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1321321/full
Počet záznamů: 1