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Gypsum endolithic phototrophs under moderate climate (Southern Sicily): their diversity and pigment composition
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SYSNO ASEP 0578330 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gypsum endolithic phototrophs under moderate climate (Southern Sicily): their diversity and pigment composition Tvůrce(i) Němečková, K. (CZ)
Mareš, Jan (MBU-M) ORCID
Procházková, L. (CZ)
Culka, A. (CZ)
Košek, F. (CZ)
Wierzchos, J. (ES)
Nedbalová, L. (CZ)
Dudák, J. (CZ)
Tymlová, V. (CZ)
Žemlička, J. (CZ)
Kust, A. (US)
Zima, J. (CZ)
Nováková, E. (CZ)
Jehlička, J. (CZ)Číslo článku 1175066 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 14, July 6 (2023)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova mcmurdo dry valleys ; raman-spectroscopy ; microbial colonization ; atacama desert ; in-situ ; scytonemin ; microorganisms ; cyanobacteria ; carotenoids ; communities ; endoliths ; gypsum ; phototrophs ; cyanobacteria ; green algae ; metagenomics Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 001032976300001 EID SCOPUS 85165259978 DOI 10.3389/fmicb.2023.1175066 Anotace In this study, we used microscopic, spectroscopic, and molecular analysis to characterize endolithic colonization in gypsum (selenites and white crystalline gypsum) from several sites in Sicily. Our results showed that the dominant microorganisms in these environments are cyanobacteria, including: Chroococcidiopsis sp., Gloeocapsopsis pleurocapsoides, Gloeocapsa compacta, and Nostoc sp., as well as orange pigmented green microalgae from the Stephanospherinia clade. Single cell and filament sequencing coupled with 16S rRNA amplicon metagenomic profiling provided new insights into the phylogenetic and taxonomic diversity of the endolithic cyanobacteria. These organisms form differently pigmented zones within the gypsum. Our metagenomic profiling also showed differences in the taxonomic composition of endoliths in different gypsum varieties. Raman spectroscopy revealed that carotenoids were the most common pigments present in the samples. Other pigments such as gloeocapsin and scytonemin were also detected in the near-surface areas, suggesting that they play a significant role in the biology of endoliths in this environment. These pigments can be used as biomarkers for basic taxonomic identification, especially in case of cyanobacteria. The findings of this study provide new insights into the diversity and distribution of phototrophic microorganisms and their pigments in gypsum in Southern Sicily. Furthemore, this study highlights the complex nature of endolithic ecosystems and the effects of gypsum varieties on these communities, providing additional information on the general bioreceptivity of these environments. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175066/full
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