Počet záznamů: 1
Normobaric hypoxia shows enhanced FOXO1 signaling in obese mouse gastrocnemius muscle linked to metabolism and muscle structure and neuromuscular innervation
- 1.
SYSNO ASEP 0576881 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Normobaric hypoxia shows enhanced FOXO1 signaling in obese mouse gastrocnemius muscle linked to metabolism and muscle structure and neuromuscular innervation Tvůrce(i) Song, J. (NL)
Duivenvoorde, L. P. M. (NL)
Grefte, S. (NL)
Kuda, Ondřej (FGU-C) RID, ORCID, SAI
Martínez-Ramírez, Felipe (FGU-C)
van der Stelt, I. (NL)
Mastorakou, D. (NL)
van Schothorst, E. M. (NL)
Keijer, J. (NL)Zdroj.dok. Pflugers Archiv - European Journal of Physiology - ISSN 0031-6768
Roč. 475, č. 11 (2023), s. 1265-1281Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova hypoxia ; skeletal muscle ; FOXO ; mitochondria ; metabolism ; neuromuscular junction Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 001060183600001 EID SCOPUS 85169313487 DOI 10.1007/s00424-023-02854-4 Anotace Skeletal muscle relies on mitochondria for sustainable ATP production, which may be impacted by reduced oxygen availability (hypoxia). Compared with long-term hypoxia, the mechanistic in vivo response to acute hypoxia remains elusive. Therefore, we aimed to provide an integrated description of the Musculus gastrocnemius response to acute hypoxia. Fasted male C57BL/6JOlaHsd mice, fed a 40en% fat diet for six weeks, were exposed to 12% O2 normobaric hypoxia or normoxia (20.9% O2) for six hours (n = 12 per group). Whole-body energy metabolism and the transcriptome response of the M. gastrocnemius were analyzed and confirmed by acylcarnitine determination and Q-PCR. At the whole-body level, six hours of hypoxia reduced energy expenditure, increased blood glucose and tended to decreased the respiratory exchange ratio (RER). Whole-genome transcriptome analysis revealed upregulation of forkhead box-O (FOXO) signalling, including an increased expression of tribbles pseudokinase 3 (Trib3). Trib3 positively correlated with blood glucose levels. Upregulated carnitine palmitoyltransferase 1A negatively correlated with the RER, but the significantly increased in tissue C14-1, C16-0 and C18-1 acylcarnitines supported that β-oxidation was not regulated. The hypoxia-induced FOXO activation could also be connected to altered gene expression related to fiber-type switching, extracellular matrix remodeling, muscle differentiation and neuromuscular junction denervation. Our results suggest that a six-hour exposure of obese mice to 12% O2 normobaric hypoxia impacts M. gastrocnemius via FOXO1, initiating alterations that may contribute to muscle remodeling of which denervation is novel and warrants further investigation. The findings support an early role of hypoxia in tissue alterations in hypoxia-associated conditions such as aging and obesity. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2024 Elektronická adresa https://doi.org/10.1007/s00424-023-02854-4
Počet záznamů: 1