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Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization
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SYSNO ASEP 0561863 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization Tvůrce(i) Masojídek, Jiří (MBU-M) RID, ORCID
Ranglová, Karolína (MBU-M) ORCID
Bečková, Martina (MBU-M) RID
Torzillo, G. (CR)
Knoppová, Jana (MBU-M) RID
Silva Benavides, A. M. (CR)
Charvát, Filip (MBU-M)
Komenda, Josef (MBU-M) RID, ORCIDZdroj.dok. Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 34, č. 5 (2022), s. 2339-2353Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Chlorophyll-protein ; Chlorophyll fluorescence ; Light-harvesting antenna size ; Microalgae ; Oxygen-production ; Photosynthesis Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP TN01000048 GA TA ČR - Technologická agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000829689600001 EID SCOPUS 85134763624 DOI 10.1007/s10811-022-02803-1 Anotace Photoacclimation of two Chlorella cultures strain g-120 characterised by a reduced size of light-harvesting antenna complex (LHC) and strain R-117 with full antenna size was studied during 5-day outdoor trials. The aim was to correlate the functional and structural changes in the photosynthetic apparatus to culture growth, photochemical activity and thylakoid composition of chlorophyll (Chl)-protein complexes and corresponding polypeptides. Chlorella g-120 was characterized by a low Chl/biomass ratio (< 0.5% of dry weight), about four times lower compared to Chlorella R-117. The important observation was that the high molecular mass Chl-binding protein supercomplexes, i.e. Photosystem II (PSII) and Photosystem I (PSI) cores associated with LHCs were physically missing or negligible in Chlorella g-120. However, there were no visible changes in Chl-protein composition in the g-120 strain during its acclimation to phototrophic conditions. Measurement of the effective absorption cross-section of PSII centres confirmed a markedly reduced functional antenna size in Chlorella g-120 as compared to R-117 which coincided with the absence of the PSII-LHC supercomplexes. We demonstrated that Chlorella g-120 represents a typical reduced antenna-size strain due to its Chl-protein composition. As compared to the full-antenna Chlorella R-117 strain, the outdoor cultures of Chlorella g-120 showed significantly lower oxygen production and electron transport rate measured in-situ. On the contrary, Chlorella g-120 revealed increased futile energy dissipation via non-photochemical quenching and higher respiration compared to Chlorella R-117. Consequently, the potential use of microalgae strains with reduced LHCII for outdoor mass cultivation may not be as straightforward as anticipated from laboratory experiments. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://link.springer.com/article/10.1007/s10811-022-02803-1
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