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Application-based guidelines for best practices in plant flow cytometry
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SYSNO ASEP 0561312 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Application-based guidelines for best practices in plant flow cytometry Tvůrce(i) Sliwinska, E. (PL)
Loureiro, J. (PT)
Leitch, I. J. (GB)
Šmarda, P. (CZ)
Bainard, J. (CA)
Bureš, P. (CZ)
Chumová, Zuzana (BU-J) ORCID, RID
Horová, L. (CZ)
Koutecký, P. (CZ)
Lučanová, Magdalena (BU-J) RID, ORCID
Trávníček, Pavel (BU-J) RID, ORCID
Galbraith, D. W. (US)Zdroj.dok. Cytometry. Part A. - : Wiley - ISSN 1552-4922
Roč. 101, č. 9 (2022), s. 749-781Poč.str. 33 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova endoreduplication ; genome size ; plant ploidy Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP GA17-18080S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BU-J - RVO:67985939 UT WOS 000700954500001 EID SCOPUS 85112747267 DOI 10.1002/cyto.a.24499 Anotace Flow cytometry (FCM) is currently the most widely-used method to establish nuclear DNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are sufficient for most plant applications, are commercially available at a reasonable price, the number of laboratories equipped with these instruments, and consequently new FCM users, has greatly increased over the last decade. This paper meets an urgent need for comprehensive recommendations for best practices in FCM for different plant science applications. We discuss advantages and limitations of establishing plant ploidy, genome size, DNA base composition, cell cycle activity, and level of endoreduplication. Applications of such measurements in plant systematics, ecology, molecular biology research, reproduction biology, tissue cultures, plant breeding, and seed sciences are described. Advice is included on how to obtain accurate and reliable results, as well as how to manage troubleshooting that may occur during sample preparation, cytometric measurements, and data handling. Each section is followed by best practice recommendations, tips as to what specific information should be provided in FCM papers are also provided. Pracoviště Botanický ústav Kontakt Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Rok sběru 2023 Elektronická adresa https://doi.org/10.1002/cyto.a.24499
Počet záznamů: 1