Počet záznamů: 1
Similarities and differences in fish community composition accessed by electrofishing, gill netting, seining, trawling, and water eDNA metabarcoding in temperate reservoirs.
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SYSNO ASEP 0559607 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Similarities and differences in fish community composition accessed by electrofishing, gill netting, seining, trawling, and water eDNA metabarcoding in temperate reservoirs. Tvůrce(i) Golpour, Amin Dehsari (BC-A) RID
Šmejkal, Marek (BC-A) RID, ORCID
Čech, Martin (BC-A) RID, ORCID
dos Santos, Romulo Acacio (BC-A)
Souza, Allan T. (BC-A) RID, ORCID
Jůza, Tomáš (BC-A) RID, ORCID
Martínez, Carlos (BC-A) RID, ORCID
Bartoň, Daniel (BC-A) RID
Vašek, Mojmír (BC-A) RID, ORCID
Draštík, Vladislav (BC-A) RID, ORCID
Kolařík, Tomáš (BC-A)
Kočvara, Luboš (BC-A) RID, ORCID
Říha, Milan (BC-A) RID, ORCID
Peterka, Jiří (BC-A) RID, ORCID
Blabolil, Petr (BC-A) RID, ORCIDCelkový počet autorů 15 Číslo článku 913279 Zdroj.dok. Frontiers in Ecology and Evolution. - : Frontiers Research Foundation - ISSN 2296-701X
Roč. 10, Jul (2022)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova biomonitoring ; environmental DNA ; reservoir ; sampling method ; species detection Vědní obor RIV EH - Ekologie - společenstva Obor OECD Ecology Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000827260700001 EID SCOPUS 85134205289 DOI 10.3389/fevo.2022.913279 Anotace It is difficult to understand the composition and diversity of biological communities in complex and heterogeneous environments using traditional sampling methods. Recently, developments in environmental DNA metabarcoding have emerged as a powerful, non-invasive method for comprehensive community characterization and biodiversity monitoring in different types of aquatic ecosystems. In this study, water eDNA targeting fish (wf-eDNA) and four traditional fish sampling methods (electrofishing, gill netting, seining, trawling) were compared to evaluate the reliability and efficiency of wf-eDNA (vertebrate mitochondrial 12S ribosomal RNA (rRNA) as an alternative approach to assess the diversity and composition of freshwater fish communities. The results of wf-eDNA showed a consistency between the traditional sampling methods regarding species detection. However, some fish species detected using wf-eDNA assay were not detected using traditional sampling methods and vice versa. Comparison of wf-eDNA and traditional sampling methods revealed spatial homogeneity in fish community composition in all reservoirs. Ordination analysis showed that the wf-eDNA approach covers all traditional sampling methods and occupies an intermediate position. In addition, based on the Shannon diversity index, we found that in one reservoir the wf-eDNA method yielded similar fish community diversity to traditional sampling methods. However, in other reservoirs, the calculated Shannon diversity index of the wf-eDNA method was significantly higher than traditional sampling methods. In general, significant positive correlations were found between the wf-eDNA method and almost all traditional sampling methods. We conclude that wf-eDNA seems to be a reliable and complementary approach for biomonitoring and ecosystem management of freshwater ichthyofauna. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://doi.org/10.3389/fevo.2022.913279
Počet záznamů: 1