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Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s
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SYSNO ASEP 0556452 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s Tvůrce(i) Urbánek, Tomáš (UMCH-V) ORCID, RID
Ivanko, Iryna (UMCH-V) RID, ORCID
Svoboda, Jan (UMCH-V) ORCID, RID
Tomšík, Elena (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCIDČíslo článku 131827 Zdroj.dok. Sensors and Actuators B - Chemical. - : Elsevier
Roč. 363, 15 July (2022)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova ROS ; polyporphyrine ; nonbiofouling Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA21-01090S GA ČR - Grantová agentura ČR NU20-06-00424 GA MZd - Ministerstvo zdravotnictví Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000806144200005 EID SCOPUS 85127821322 DOI 10.1016/j.snb.2022.131827 Anotace We built a robust selective potentiometric sensor of reactive oxygen species (ROS) as a thin layer applied on the surface of smart implants, which enables the detection and localization of inflammation at an early stage. The sensor consists of a conductive polymer layer based on polythiophene with an incorporated porphyrin-metal complex that potentiometrically detects the presence of ROS. This sensor is covalently coated with a nonbiofouling layer of poly(2-methyl-2-oxazoline), which works as a biocompatibilizer but mainly prevents the sorption of proteins and other biomacromolecules naturally occurring in organisms, which could interfere with the ROS signal. We have shown that our potentiometric sensor shows a rapid response to hydrogen peroxide, does not experience interference with bovine serum albumin as a model serum protein when sensing ROS, is able to fully reversibly detect ROS with a linear response within a very wide range of biologically relevant concentrations and, most importantly, is able to distinguish between hydrogen peroxide and hypochlorite. We also performed a head-to-head comparison of two positional isomers of thienylated porphyrine for sensor applications (2TTP and 3TTP, with 3TTP shown to be more appropriate) and four different coordinated metals (Cu, Fe, Co and Mn, with Cu and especially Fe shown to be the most appropriate). Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0925400522004695?via%3Dihub
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