Počet záznamů: 1
A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont
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SYSNO ASEP 0555255 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont Tvůrce(i) Zakharova, A. (CZ)
Saura, A. (CZ)
Butenko, Anzhelika (BC-A) RID, ORCID
Podešvová, L. (CZ)
Warmusova, S. (CZ)
Kostygov, A.Y. (CZ)
Nenarokova, Anna (BC-A) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCID
Opperdoes, F. R. (BE)
Yurchenko, V. (CZ)Celkový počet autorů 10 Číslo článku e01606-21 Zdroj.dok. mBio. - : American Society for Microbiology - ISSN 2161-2129
Roč. 12, č. 4 (2021)Poč.str. 18 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova insect trypanosomatids ; symbiotic bacterium ; strigomonas-culicis ; crithidia-deanei ; evolution ; leishmania ; metabolism ; parasite ; expression ; alignment ; Trypanosomatidae ; Leishmaniinae ; bacterial endosymbiont ; genomics ; metabolism Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA20-07186S GA ČR - Grantová agentura ČR GA21-09283S GA ČR - Grantová agentura ČR LL1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000696627000007 EID SCOPUS 85114296400 DOI 10.1128/mBio.01606-21 Anotace The closest relative of human pathogen Leishmania, the trypanosomatid Novymonas esmeraldas, harbors a bacterial endosymbiont Candidatus Pandoraea novymonadis. Based on genomic data, we performed a detailed characterization of the metabolic interactions of both partners. While in many respects the metabolism of N. esmeraldas resembles that of other Leishmaniinae, the endosymbiont provides the trypanosomatid with heme, essential amino acids, purines, some coenzymes, and vitamins. In return, N. esmeraldas shares with the bacterium several nonessential amino acids and phospholipids. Moreover, it complements its carbohydrate metabolism and urea cycle with enzymes missing from the Ca. Pandoraea novymonadis genome. The removal of the endosymbiont from N. esmeraldas results in a significant reduction of the overall translation rate, reduced expression of genes involved in lipid metabolism and mitochondrial respiratory activity, and downregulation of several aminoacyl-tRNA synthetases, enzymes involved in the synthesis of some amino acids, as well as proteins associated with autophagy. At the same time, the genes responsible for protection against reactive oxygen species and DNA repair become signifi- cantly upregulated in the aposymbiotic strain of this trypanosomatid. By knocking out a component of its flagellum, we turned N. esmeraldas into a new model trypanosomatid that is amenable to genetic manipulation using both conventional and CRISPR-Cas9-mediated approaches. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://journals.asm.org/doi/10.1128/mBio.01606-21
Počet záznamů: 1