Počet záznamů: 1
Overexpression of Isl1 under the Pax2 Promoter, Leads to Impaired Sound Processing and Increased Inhibition in the Inferior Colliculus
- 1.
SYSNO ASEP 0549424 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Overexpression of Isl1 under the Pax2 Promoter, Leads to Impaired Sound Processing and Increased Inhibition in the Inferior Colliculus Tvůrce(i) Chumak, Tetyana (UEM-P)
Tóthová, Diana (UEM-P)
Filova, Iva (BTO-N) ORCID
Bureš, Zbyněk (UEM-P) RID, ORCID
Popelář, Jiří (UEM-P) RID
Pavlínková, Gabriela (BTO-N) RID, ORCID
Syka, Josef (UEM-P) RIDČíslo článku 4507 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 9 (2021)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova transcription factor ISL1 ; auditory system ; sound processing ; inferior colliculus ; inhibition Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology Vědní obor RIV – spolupráce Biotechnologický ústav - Genetika a molekulární biologie CEP GA20-06927S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 ; BTO-N - RVO:86652036 UT WOS 000650407500001 EID SCOPUS 85104621621 DOI 10.3390/ijms22094507 Anotace The LIM homeodomain transcription factor ISL1 is essential for the different aspects of neuronal development and maintenance. In order to study the role of ISL1 in the auditory system, we generated a transgenic mouse (Tg) expressing Isl1 under the Pax2 promoter control. We previously reported a progressive age-related decline in hearing and abnormalities in the inner ear, medial olivocochlear system, and auditory midbrain of these Tg mice. In this study, we investigated how Isl1 overexpression affects sound processing by the neurons of the inferior colliculus (IC). We recorded extracellular neuronal activity and analyzed the responses of IC neurons to broadband noise, clicks, pure tones, two-tone stimulation and frequency-modulated sounds. We found that Tg animals showed a higher inhibition as displayed by two-tone stimulation, they exhibited a wider dynamic range, lower spontaneous firing rate, longer first spike latency and, in the processing of frequency modulated sounds, showed a prevalence of high-frequency inhibition. Functional changes were accompanied by a decreased number of calretinin and parvalbumin positive neurons, and an increased expression of vesicular GABA/glycine transporter and calbindin in the IC of Tg mice, compared to wild type animals. The results further characterize abnormal sound processing in the IC of Tg mice and demonstrate that major changes occur on the side of inhibition. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/9/4507
Počet záznamů: 1