Počet záznamů: 1  

Overexpression of Isl1 under the Pax2 Promoter, Leads to Impaired Sound Processing and Increased Inhibition in the Inferior Colliculus

  1. 1.
    SYSNO ASEP0549424
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevOverexpression of Isl1 under the Pax2 Promoter, Leads to Impaired Sound Processing and Increased Inhibition in the Inferior Colliculus
    Tvůrce(i) Chumak, Tetyana (UEM-P)
    Tóthová, Diana (UEM-P)
    Filova, Iva (BTO-N) ORCID
    Bureš, Zbyněk (UEM-P) RID, ORCID
    Popelář, Jiří (UEM-P) RID
    Pavlínková, Gabriela (BTO-N) RID, ORCID
    Syka, Josef (UEM-P) RID
    Číslo článku4507
    Zdroj.dok.International Journal of Molecular Sciences. - : MDPI
    Roč. 22, č. 9 (2021)
    Poč.str.17 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovatranscription factor ISL1 ; auditory system ; sound processing ; inferior colliculus ; inhibition
    Vědní obor RIVFH - Neurologie, neurochirurgie, neurovědy
    Obor OECDNeurosciences (including psychophysiology
    Vědní obor RIV – spolupráceBiotechnologický ústav - Genetika a molekulární biologie
    CEPGA20-06927S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaUEM-P - RVO:68378041 ; BTO-N - RVO:86652036
    UT WOS000650407500001
    EID SCOPUS85104621621
    DOI10.3390/ijms22094507
    AnotaceThe LIM homeodomain transcription factor ISL1 is essential for the different aspects of neuronal development and maintenance. In order to study the role of ISL1 in the auditory system, we generated a transgenic mouse (Tg) expressing Isl1 under the Pax2 promoter control. We previously reported a progressive age-related decline in hearing and abnormalities in the inner ear, medial olivocochlear system, and auditory midbrain of these Tg mice. In this study, we investigated how Isl1 overexpression affects sound processing by the neurons of the inferior colliculus (IC). We recorded extracellular neuronal activity and analyzed the responses of IC neurons to broadband noise, clicks, pure tones, two-tone stimulation and frequency-modulated sounds. We found that Tg animals showed a higher inhibition as displayed by two-tone stimulation, they exhibited a wider dynamic range, lower spontaneous firing rate, longer first spike latency and, in the processing of frequency modulated sounds, showed a prevalence of high-frequency inhibition. Functional changes were accompanied by a decreased number of calretinin and parvalbumin positive neurons, and an increased expression of vesicular GABA/glycine transporter and calbindin in the IC of Tg mice, compared to wild type animals. The results further characterize abnormal sound processing in the IC of Tg mice and demonstrate that major changes occur on the side of inhibition.
    PracovištěÚstav experimentální medicíny
    KontaktLenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
    Rok sběru2022
    Elektronická adresahttps://www.mdpi.com/1422-0067/22/9/4507
Počet záznamů: 1  

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