Počet záznamů: 1
Complex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads
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SYSNO ASEP 0548344 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Complex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads Tvůrce(i) Vondrak, Tihana (BC-A) RID, ORCID
Oliveira, Ludmila (BC-A) RID, ORCID
Novák, Petr (BC-A) RID, ORCID
Koblížková, Andrea (BC-A)
Neumann, Pavel (BC-A) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAICelkový počet autorů 6 Zdroj.dok. Computational and Structural Biotechnology Journal. - : Elsevier - ISSN 2001-0370
Roč. 19, Apr 22 (2021), s. 2179-2189Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. SE - Švédsko Klíč. slova Satellite DNA ; Heterochromatin ; Oxford Nanopore sequencing ; Fluorescence in situ hybridization ; Holocentric chromosomes ; LINE elements Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GA20-24252S GA ČR - Grantová agentura ČR LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura ELIXIR-CZ II - 90131 - Ústav organické chemie a biochemie AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000684934600009 EID SCOPUS 85104729200 DOI https://doi.org/10.1016/j.csbj.2021.04.011 Anotace Repeat-rich regions of higher plant genomes are usually associated with constitutive heterochromatin, a specific type of chromatin that forms tightly packed nuclear chromocenters and chromosome bands. There is a large body of cytogenetic evidence that these chromosome regions are often composed of tandemly organized satellite DNA. However, comparatively little is known about the sequence arrangement within heterochromatic regions, which are difficult to assemble due to their repeated nature. Here, we explore long-range sequence organization of heterochromatin regions containing the major satellite repeat CUS-TR24 in the holocentric plant Cuscuta europaea. Using a combination of ultra-long read sequencing with assembly-free sequence analysis, we reveal the complex structure of these loci, which are composed of short arrays of CUS-TR24 interrupted frequently by emerging simple sequence repeats and targeted insertions of a specific lineage of LINE retrotransposons. These data suggest that the organization of satellite repeats constituting heterochromatic chromosome bands can be more complex than previously envisioned, and demonstrate that heterochromatin organization can be efficiently investigated without the need for genome assembly. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2001037021001227?via%3Dihub
Počet záznamů: 1