Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

0546629 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Harshkova, D. - Liakh, I. - Bialevich, Vitali - Ondrejmišková, Kamila - Aksmann, A. - Bišová, Kateřina
Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii.
Cells. Roč. 10, č. 8 (2021), č. článku 1936. E-ISSN 2073-4409
Grant CEP: GA ČR(CZ) GA19-12607S
Institucionální podpora: RVO:61388971
Klíčová slova: non-steroidal anti-inflammatory drug * diclofenac * cell cycle * Chlamydomonas reinhardtii
Obor OECD: Microbiology
Impakt faktor: 7.666, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2073-4409/10/8/1936

The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.
Trvalý link: http://hdl.handle.net/11104/0323077