Počet záznamů: 1  

Structurally Redesigned Bioorthogonal Reagents for Mitochondria-Specific Prodrug Activation

  1. 1.
    0538814 - ÚOCHB 2022 RIV US eng J - Článek v odborném periodiku
    Dzijak, Rastislav - Galeta, Juraj - Vázquez, Arcadio - Kozák, Jaroslav - Matoušová, Marika - Fulka, Helena - Dračínský, Martin - Vrábel, Milan
    Structurally Redesigned Bioorthogonal Reagents for Mitochondria-Specific Prodrug Activation.
    JACS Au. Roč. 1, č. 1 (2021), s. 23-30. E-ISSN 2691-3704
    Grant CEP: GA ČR(CZ) GA19-13811S
    GRANT EU: European Commission(XE) 677465 - SWEETOOLS
    Institucionální podpora: RVO:61388963 ; RVO:68378041
    Klíčová slova: bioorthogonal reactions * click-to-release * mitochondria * prodrug activation * tetrazines
    Obor OECD: Organic chemistry; Medicinal chemistry (UEM-P)
    Způsob publikování: Open access
    https://doi.org/10.1021/jacsau.0c00053

    The development of abiotic chemical reactions that can be performed in anorganelle-specific manner can provide new opportunities in drug delivery and cell and chemicalbiology. However, due to the complexity of the cellular environment, this remains a significantchallenge. Here, we introduce structurally redesigned bioorthogonal tetrazine reagents thatspontaneously accumulate in mitochondria of live mammalian cells. The attributes leading totheir efficient accumulation in the organelle were optimized to include the right combinationof lipophilicity and positive delocalized charge. The best performing mitochondriotropictetrazines enable subcellular chemical release of TCO-caged compounds as we show usingfluorogenic substrates and mitochondrial uncoupler niclosamide. Our work demonstrates thata shrewd redesign of common bioorthogonal reagents can lead to their transformation intoorganelle-specific probes, opening the possibility to activate prodrugs and m anipulatebiological processes at the subcellular level by using purely chemical tools.
    Trvalý link: http://hdl.handle.net/11104/0316967

     
     
Počet záznamů: 1  

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