Počet záznamů: 1
Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
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SYSNO ASEP 0537796 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model Tvůrce(i) Vašíček, Ondřej (BFU-R) ORCID, RID
Hájek, Jan (MBU-M) ORCID
Blahová, L. (CZ)
Hrouzek, Pavel (MBU-M) ORCID
Babica, Pavel (BU-J) RID, ORCID
Kubala, Lukáš (BFU-R) RID, ORCID
Šindlerová, Lenka (BFU-R) RID, ORCIDCelkový počet autorů 7 Číslo článku 101849 Zdroj.dok. Harmful Algae. - : Elsevier - ISSN 1568-9883
Roč. 96, Jun 2020 (2020)Poč.str. 8 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cell ; peptides ; proliferation ; stabilization ; toxicity ; claudins ; health Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Mikrobiologický ústav - Hydrologie a limnologie
Botanický ústav - Mikrobiologie, virologieCEP GA16-24949S GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 ; MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000541912700014 EID SCOPUS 85086601564 DOI 10.1016/j.hal.2020.101849 Anotace Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://doi.org/10.1016/j.hal.2020.101849
Počet záznamů: 1