Počet záznamů: 1
Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection
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SYSNO ASEP 0519392 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection Tvůrce(i) Tůma, P. (CZ)
Gojda, J. (CZ)
Sommerová, B. (CZ)
Koval, Dušan (UOCHB-X) RID, ORCIDČíslo článku 113772 Zdroj.dok. Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 857, Jan 15 (2020)Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova branched chain amino acids ; clinical analysis ; capillary electrophoresis ; coating ; contactless conductivity detection Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA17-12648S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000518494300030 EID SCOPUS 85076996162 DOI 10.1016/j.jelechem.2019.113772 Anotace Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 · 10−9 m2 V−1 s−1 in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min, the limits of detections are 0.13–0.14 μmol/L for the treated sample and 0.50–0.58 μmol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub
Počet záznamů: 1