Počet záznamů: 1
Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking
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SYSNO ASEP 0518889 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking Tvůrce(i) Saurav, Kumar (MBU-M) ORCID
Macho, Markéta (MBU-M) ORCID
Kust, Andreja (MBU-M) ORCID
Delawská, Kateřina (MBU-M) ORCID
Hájek, Jan (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCIDZdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 64, č. 5 (2019), s. 645-654Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova peptidic natural-products ; drug discovery ; database search Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GJ19-17868Y GA ČR - Grantová agentura ČR Výzkumná infrastruktura C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000496409500005 EID SCOPUS 85070227618 DOI 10.1007/s12223-019-00737-9 Anotace The rapid emergence of resistance in pathogenic bacteria together with a steep decline in economic incentives has rendered a new wave in the drug development by the pharmaceutical industry and researchers. Since cyanobacteria are recognized as wide producers of pharmaceutically important compounds, we investigated thirty-four cyanobacterial extracts prepared by solvents of different polarities for their antimicrobial potential. Almost all tested cyanobacterial strains exhibited some degree of antimicrobial bioactivity, with more general effect on fungal strains compared with bacteria. Surprisingly ~50% of cyanobacterial extracts exhibited specific activity against one or few bacterial indicator strains with Gram-positive bacteria being more affected. Extracts of two most promising strains were subjected to activity-guided fractionation and determination of the minimum inhibitory concentration (MIC) against selected bacterial and fungal isolates. Multiple fractions were responsible for their antimicrobial effect with MIC reaching low-micromolar concentrations and in some of them high level of specificity was recorded. Twenty-six bioactive fractions analyzed on LC-HRMS/MS and Global Natural Product Social Molecular Networking (GNPS) online workflow using dereplication resulted in identification of only forty-nine peptide spectrum matches (PSMs) with eleven unique metabolites spectrum matches (MSMs). Interestingly, only three fractions from Nostoc calcicola Lukesova 3/97 and four fractions from Desmonostoc sp. Cc2 showed the presence of unique MSMs suggesting the presence of unknown antimicrobial metabolites among majority of bioactive fractions from both the strains. Our results highlight potential for isolation and discovery of potential antimicrobial bioactive lead molecules from cyanobacterial extracts. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://link.springer.com/article/10.1007%2Fs12223-019-00737-9
Počet záznamů: 1