Počet záznamů: 1  

Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene

  1. 1.
    0504183 - ÚEB 2020 RIV DE eng J - Článek v odborném periodiku
    Janáková, Eva - Jakobson, I. - Peusha, H. - Abrouk, Michael - Škopová, Monika - Šimková, Hana - Šafář, Jan - Vrána, Jan - Doležel, Jaroslav - Järve, K. - Valárik, Miroslav
    Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene.
    Theoretical and Applied Genetics. Roč. 132, č. 4 (2019), s. 1061-1072. ISSN 0040-5752. E-ISSN 1432-2242
    Grant CEP: GA MŠMT(CZ) LO1204; GA ČR(CZ) GA18-11688S; GA MZe QK1710302
    Institucionální podpora: RVO:61389030
    Klíčová slova: Cloning * Food products * Fungi
    Obor OECD: Plant sciences, botany
    Impakt faktor: 4.439, rok: 2019
    Způsob publikování: Open access
    http://doi.org/10.1007/s00122-018-3259-3

    A segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments’ sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.
    Trvalý link: http://hdl.handle.net/11104/0295901

     
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Počet záznamů: 1  

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