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Pressure assisted partial filling affinity capillary electrophoresis employed for determination of binding constants of human insulin hexamer complexes with serotonin, dopamine, arginine, and phenol
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SYSNO ASEP 0501552 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Pressure assisted partial filling affinity capillary electrophoresis employed for determination of binding constants of human insulin hexamer complexes with serotonin, dopamine, arginine, and phenol Tvůrce(i) Šolínová, Veronika (UOCHB-X) RID, ORCID
Žáková, Lenka (UOCHB-X) RID, ORCID
Jiráček, Jiří (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1052, Apr 4 (2019), s. 170-178Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova affinity capillary electrophoresis ; binding constant ; human insulin ; insulin hexamer ; neurotransmitters ; partial filling Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA17-10832S GA ČR - Grantová agentura ČR GA18-02597S GA ČR - Grantová agentura ČR EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000456436100019 EID SCOPUS 85057043367 DOI 10.1016/j.aca.2018.11.026 Anotace A new method, pressure assisted partial filling affinity capillary electrophoresis, has been developed to study noncovalent molecular interactions of the hexamer of human insulin (HI) with biologically relevant ligands, basic phenolic neurotransmitters serotonin and dopamine, basic amino acid arginine, and very weakly acidic phenol, in alkaline aqueous media. The apparent binding constants, K-b, of the HI-ligand complexes were determined from the dependence of the effective migration time changes of the above ligands on the variable zone lengths of HI hexamer dissolved in the background electrolyte (BGE) and hydrodynamically introduced into the bare fused silica capillary close to the UV detector. The strong cationic electroosmotic flow (EOF) in alkaline BGEs, 40/40 mM Tris/tricine, pH 8.1, and 25/34 mM NaOH/tricine, pH 8.5, with EOF mobilities 52.0 x 10(-9) and 58.0 x 10(-9) m(2)V(-1)s(-1), respectively, was reduced by the hydrodynamic counter flow induced by external pressure at the outlet capillary end to avoid expulsion of HI zone out of the capillary and to allow HI interaction with both cationic and anionic ligands inside the capillary. The HI hexamer interactions with the above ligands were found to be weak to moderately strong, with K-b values in the range 385-1314 L mol(-1), and decreasing in the order HIphenol > HI-dopamine > HI-serotonin > HI-arginine. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0003267018313655?via%3Dihub
Počet záznamů: 1