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Tick galactosyltransferases are involved in alpha-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development
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SYSNO ASEP 0497760 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tick galactosyltransferases are involved in alpha-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development Tvůrce(i) Cabezas Cruz, Alejandro (BC-A)
Espinosa, P. J. (ES)
Alberdi, P. (ES)
Šimo, L. (FR)
Valdés, James J. (BC-A) RID, ORCID
Mateos-Hernández, L. (ES)
Contreras, M. (ES)
Villar Rayo, M. (ES)
de la Fuente, J. (ES)Celkový počet autorů 9 Číslo článku 14224 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 8, September 21 (2018)Poč.str. 18 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova red meat allergy ; multiple sequence alignment ; human gb3/cd77 synthase ; amino-acid ; pathogen infection ; metabolic pathways ; immune-response ; cell-lines ; expression ; identification Vědní obor RIV EC - Imunologie Obor OECD Immunology Institucionální podpora BC-A - RVO:60077344 UT WOS 000445276500005 EID SCOPUS 85053724174 DOI 10.1038/s41598-018-32664-z Anotace The carbohydrate Gal alpha 1-3Gal beta 1-(3)4GlcNAc-R (alpha-Gal) is produced in all mammals except for humans, apes and old world monkeys that lost the ability to synthetize this carbohydrate. Therefore, humans can produce high antibody titers against alpha-Gal. Anti-alpha-Gal IgE antibodies have been associated with tick-induced allergy (i.e. alpha-Gal syndrome) and anti-alpha-Gal IgG/IgM antibodies may be involved in protection against malaria, leishmaniasis and Chagas disease. The alpha-Gal on tick salivary proteins plays an important role in the etiology of the alpha-Gal syndrome. However, whether ticks are able to produce endogenous alpha-Gal remains currently unknown. In this study, the Ixodes scapularis genome was searched for galactosyltransferases and three genes were identified as potentially involved in the synthesis of alpha-Gal. Heterologous gene expression in alpha-Gal-negative cells and gene knockdown in ticks confirmed that these genes were involved in alpha-Gal synthesis and are essential for tick feeding. Furthermore, these genes were shown to play an important role in tick-pathogen interactions. Results suggested that tick cells increased alpha-Gal levels in response to Anaplasma phagocytophilum infection to control bacterial infection. These results provided the molecular basis of endogenous alpha-Gal production in ticks and suggested that tick galactosyltransferases are involved in vector development, tick-pathogen interactions and possibly the etiology of alpha-Gal syndrome in humans. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2019 Elektronická adresa https://www.nature.com/articles/s41598-018-32664-z
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