Počet záznamů: 1
Single molecule upconversion-linked immunosorbent assay with extended dynamic range for the sensitive detection of diagnostic biomarkers
- 1.
SYSNO ASEP 0481051 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Single molecule upconversion-linked immunosorbent assay with extended dynamic range for the sensitive detection of diagnostic biomarkers Tvůrce(i) Farka, Z. (CZ)
Matthias, J. M. (DE)
Hlaváček, Antonín (UIACH-O) ORCID
Skládal, P. (CZ)
Gorris, H H. (DE)Celkový počet autorů 5 Zdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 89, NOV (2017), s. 11825-11830Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova photon upconversion ; immunoassay ; single molecule detection Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GBP206/12/G014 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000414887000093 EID SCOPUS 85043459122 DOI 10.1021/acs.analchem.7b03542 Anotace The ability to detect disease markers at the single molecule level promises
the ultimate sensitivity in clinical diagnosis. Fluorescence-based single-molecule analysis,
however, is limited by matrix interference and can only probe a very small detection
volume, which is typically not suitable for real world analytical applications. We have
developed a microtiter plate immunoassay for counting single molecules of the cancer
marker prostate specific antigen (PSA) using photon-upconversion nanoparticles
(UCNPs) as labels that can be detected without background fluorescence. Individual
sandwich immunocomplexes consisting of (1) an anti-PSA antibody immobilized to the
surface of a microtiter well, (2) PSA, and (3) an anti-PSA antibody-UCNP conjugate were
counted under a wide-field epifluorescence microscope equipped with a 980 nm laser
excitation source. The single-molecule (digital) upconversion-linked immunosorbent assay
(ULISA) reaches a limit of detection of 1.2 pg mL−1 (42 fM) PSA in 25% blood serum,
which is about ten times more sensitive than commercial ELISAs, and covers a dynamic
range of three orders of magnitude. This upconversion detection mode has the potential
to pave the way for a new generation of digital immunoassays.Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2018
Počet záznamů: 1