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The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light
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SYSNO ASEP 0480474 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light Tvůrce(i) Kumar, D. (CZ)
Kvíderová, J. (CZ)
Kaštánek, P. (CZ)
Lukavský, Jaromír (BU-J) RID, ORCIDZdroj.dok. Engineering in Life Sciences. - : Wiley - ISSN 1618-0240
Roč. 17, č. 9 (2017), s. 1030-1038Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Dictyosphaerium chlorelloides ; Biomass ; Crossed gradients Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials CEP TE01020080 GA TA ČR - Technologická agentura ČR Institucionální podpora BU-J - RVO:67985939 UT WOS 000412291600008 EID SCOPUS 85020440120 DOI 10.1002/elsc.201700014 Anotace The green microalga Dictyosphaerium chlorelloides was identified as promising microorganism for biotechnological production of exopolysaccharides (EPS). In stationary phase the culture suspension solidifies to thick gel, with very high viscosity and high content of EPS which may be interesting for many biotechnological applications. To develop cultivation protocol for maximum biomass/polysaccharide production, the optimum conditions for growth and polysaccharides production were determined in this study using the crossed gradient cultivation method. Temperature and irradiance requirements of Dictyosphaerium chlorelloides were evaluated by statistical analyses for growth rate/biomass, extracellular (EPS) and intracellular (IPS) polysaccharides contents in crossed gradients of temperature (4–45°C) and irradiance (2–18W/m2, 9.1 – 82.3 μmol/(m2 s)). The maximum relative growth rate was observed at temperatures around 19.2°C and relatively low irradiances in range 2.6–11W/m2 (11.9–50.3μmol/(m2 s)). The maximumIPS production was observed at temperatures around 19.2°C and irradiance around 11 W/m2 (50.3 μmol/(m2 s)). The maximum production of EPS was observed at temperatures around 25.7°C and similar irradiances as IPS production. Due to temperature separation of growth and EPS production, development of cultivation protocol based controlled temperature manipulation is possible. Pracoviště Botanický ústav Kontakt Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Rok sběru 2018
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