Počet záznamů: 1
Impact of novel palmitoylated prolactin-releasing peptide analogs on metabolic changes in mice with diet-induced obesity
- 1.
SYSNO ASEP 0480097 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of novel palmitoylated prolactin-releasing peptide analogs on metabolic changes in mice with diet-induced obesity Tvůrce(i) Pražienková, V. (CZ)
Holubová, M. (CZ)
Pelantová, H. (CZ)
Bugáňová, M. (CZ)
Pirník, Z. (SK)
Mikulášková, Barbora (FGU-C)
Popelová, A. (CZ)
Blechová, M. (CZ)
Haluzík, M. (CZ)
Železná, B. (CZ)
Kuzma, M. (CZ)
Kuneš, Jaroslav (FGU-C) RID, ORCID
Maletínská, L. (CZ)Číslo článku e0183449 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 8 (2017)Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova food intake regulation ; peroxisome proliferation ; lipidized analogs Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP GA15-08679S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000408010000024 EID SCOPUS 85027853200 DOI 10.1371/journal.pone.0183449 Anotace Analogs of anorexigenic neuropeptides, such as prolactin-releasing peptide (PrRP), have a potential as new anti-obesity drugs. In our previous study, palmitic acid attached to the N-terminus of PrRP enabled its central anorexigenic effects after peripheral administration. In this study, two linkers, gamma-glutamic acid at Lys11 and a short, modified polyethylene glycol at the N-terminal Ser and/or Lys11, were applied for the palmitoylation of PrRP31 to improve its bioavailability. These analogs had a high affinity and activation ability to the PrRP receptor GPR10 and the neuropeptide FF2 receptor, as well as short-term anorexigenic effect similar to PrRP palmitoylated at the N-terminus. Two-week treatment with analogs that were palmitoylated through linkers to Lys11 (analogs 1 and 2), but not with analog modified both at the N-terminus and Lys11 (analog 3) decreased body and liver weights, insulin, leptin, triglyceride, cholesterol and free fatty acid plasma levels in a mouse model of diet-induced obesity. Moreover, the expression of uncoupling protein-1 was increased in brown fat suggesting an increase in energy expenditure. In addition, treatment with analogs 1 and 2 but not analog 3 significantly decreased urinary concentrations of 1-methylnicotinamide and its oxidation products N-methyl-2-pyridone-5-carboxamide and N-methyl-4-pyridone-3-carboxamide, as shown by NMR-based metabolomics. This observation confirmed the previously reported increase in nicotinamide derivatives in obesity and type 2 diabetes mellitus and the effectiveness of analogs 1 and 2 in the treatment of these disorders. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1